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Isolation and characterization of 16 microsatellite markers in Intsia palembanica, a high-value tropical hardwood species

  • Microsatellite Letters
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Abstract

Intsia palembanica is a high-value tropical hardwood species of the family Leguminosae. A total of 16 microsatellite markers were developed for this important timber species using an enrichment approach. These markers were screened on 24 samples from a natural population and subsequently tested for reproducibility, ease of scoring and locus-specificity, across five populations. The observed and expected heterozygosities ranged from 0.026 to 0.976 and 0.026 to 0.928, respectively, while the mean number of alleles per locus per population was 8.73. These microsatellite markers are informative and will be useful for conservation genetic study, individual identification and timber tracking.

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Acknowledgments

The authors thank Mariam Din, Sharifah Talib, Ramli Ponyoh, Ghazali Jaafar, Yahya Marhani, Suryani Che Seman, Nurul Hudaini Mamat and Rajaletchumi Chanderan Sagaran for their excellent assistance in the laboratory and at the field. The Kedah, Perak, Selangor and Negeri Sembilan State Forest Departments are acknowledged for granting permission to collect samples from the forest. This Project was funded by the Ministry of Natural Resources and Environment, Malaysia, under the 10th Malaysia Plan.

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Authors and Affiliations

  1. Genetics Laboratory, Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia

    Chai Ting Lee, Soon Leong Lee, Lee Hong Tnah, Kevin Kit Siong Ng, Chin Hong Ng & Shawn Cheng

  2. Forestry Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan

    Naoki Tani

Authors
  1. Chai Ting Lee
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  2. Soon Leong Lee
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  3. Lee Hong Tnah
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  4. Kevin Kit Siong Ng
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  5. Chin Hong Ng
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  6. Shawn Cheng
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  7. Naoki Tani
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Correspondence to Chai Ting Lee.

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Lee, C.T., Lee, S.L., Tnah, L.H. et al. Isolation and characterization of 16 microsatellite markers in Intsia palembanica, a high-value tropical hardwood species. Conservation Genet Resour 6, 389–391 (2014). https://doi.org/10.1007/s12686-013-0100-9

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  • DOI: https://doi.org/10.1007/s12686-013-0100-9

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