Abstract
Seventeen polymorphic microsatellite loci were isolated and characterized for Coilia nasus. The number of alleles per locus ranged from 3 to 18, and the observed and expected heterozygosity ranged from 0.3333 to 0.9583 and from 0.3032 to 0.9111, respectively. These markers will be useful for further studies on the genetic diversity and population structure of this species.
References
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222
Liu JX, Avise JC (2011) High degree of multiple paternity in the viviparous Shiner Perch, Cymatogaster aggregata, a fish with long-term female sperm storage. Mar Biol 158:893–901
Ma CY, Cheng QQ, Zhang QY, Zhuang P, Zhao YL (2010) Genetic variation of Coilia ectenes (Clupeiformes: Engraulidae) revealed by the complete cytochrome b sequences of mitochondrial DNA. J Exp Mar Biol Ecol 385:14–19
Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8(1):103–106
van Oosterhout C, Hutchinson WF, Wills DP, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4(3):535–538
Acknowledgments
The study was supported by the Grants from NSF China Grant (No. 41121064) and 100 Talents Program of the Chinese Academy of Sciences to JXL, and Fundamental Research Funds for the Central Universities (201262022).
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Yang, Q., Gao, T. & Liu, JX. Development and characterization of 17 microsatellite loci in an anadromous fish Coilia nasus . Conservation Genet Resour 6, 357–359 (2014). https://doi.org/10.1007/s12686-013-0093-4
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DOI: https://doi.org/10.1007/s12686-013-0093-4