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Development of 27 trinucleotide microsatellite markers for Saccharina japonica using next generation sequencing technology

  • Microsatellite Letters
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Abstract

Twenty-seven polymorphic trinucleotide microsatellite DNA markers were developed from Saccharina japonica, through paired-end Illumina sequencing data. Sixty-three gametophyte clones of the species, preserved indoor as germplasm resources were genotyped using these markers. The number of alleles ranged from 2 to 7 with an average 4.7 per locus. The gene diversity and expected heterozygosity varied from 0.171 to 0.774 and from 0.174 to 0.793, respectively. Sixteen loci could be successfully amplified in Saccharina longissima with 12 loci being polymorphic. These novel microsatellite DNA markers will be extremely useful in germplasm resource conservation and management of S. japonica and also in its related species S. longissima.

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Acknowledgments

This study was financially supported by the National High-Tech R&D Program of China (2012AA10A406) and National Key Technology R&D Support Program of China (2012BAD55G01).

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Authors and Affiliations

  1. National Algae Engineering and Technique Research Center, Shandong Oriental Ocean Sci-tech Co. Ltd, Yantai, 264003, Shandong, People’s Republic of China

    Linan Zhang, Jie Peng, Xiaojie Li, Yanling Liu, Cuiju Cui, Ruina Wu, Pingping Tian & Yan Li

  2. Qingdao Agriculture University, Qingdao, 266109, Shandong, People’s Republic of China

    Hao Wu

Authors
  1. Linan Zhang
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  2. Jie Peng
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  3. Xiaojie Li
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  4. Yanling Liu
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  5. Cuiju Cui
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  6. Hao Wu
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  7. Ruina Wu
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  8. Pingping Tian
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  9. Yan Li
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Corresponding author

Correspondence to Hao Wu.

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Zhang, L., Peng, J., Li, X. et al. Development of 27 trinucleotide microsatellite markers for Saccharina japonica using next generation sequencing technology. Conservation Genet Resour 6, 341–344 (2014). https://doi.org/10.1007/s12686-013-0089-0

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  • DOI: https://doi.org/10.1007/s12686-013-0089-0

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