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Conservation Genetics Resources

, Volume 6, Issue 2, pp 337–339 | Cite as

Isolation and characterization of 13 polymorphic microsatellite loci for the smooth Cherax cainii and hairy marron C. tenuimanus (Decapoda: Parastacidae)

  • W. Jason Kennington
  • Clodagh Guildea
  • Sherralee S. Lukehurst
  • Yvette Hitchen
  • Michael G. Gardner
  • Rodney Duffy
  • P. Joana Dias
  • Jason M. Ledger
  • Michael Snow
Microsatellite Letters

Abstract

Numbers of the critically endangered hairy marron Cherex tenuimanus are declining rapidly in its natural habitat, mainly due to competition with smooth marron C. cainii. To determine whether genetic introgression is occurring between the two species we isolated 13 new polymorphic microsatellite markers from C. cainii using 454 shotgun sequencing. The loci were screened for variation in 20 individuals of C. cainii and eight individuals of C. tenuimanus. There was no evidence of linkage disequilibrium between pairs of loci and only one locus deviated significantly from Hardy–Weinberg equilibrium. The number of alleles per locus ranged from two to 11 across both species.

Keywords

Cherex cainii Cherex tenuimanus Marron Microsatellites Freshwater crayfish 

Notes

Acknowledgments

We acknowledge the assistance and support of Alison Fitch from Flinders University. This project was funded by the Western Australian Department of Fisheries.

References

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • W. Jason Kennington
    • 1
  • Clodagh Guildea
    • 1
  • Sherralee S. Lukehurst
    • 1
  • Yvette Hitchen
    • 2
  • Michael G. Gardner
    • 3
    • 4
    • 5
  • Rodney Duffy
    • 6
  • P. Joana Dias
    • 1
    • 6
  • Jason M. Ledger
    • 7
  • Michael Snow
    • 6
  1. 1.Centre for Evolutionary Biology, School of Animal BiologyThe University of Western AustraliaCrawleyAustralia
  2. 2.Helix Molecular SolutionsLeedervilleAustralia
  3. 3.School of Biological SciencesFlinders UniversityAdelaideAustralia
  4. 4.Australian Centre for Evolutionary Biology and BiodiversityUniversity of AdelaideAdelaideAustralia
  5. 5.Evolutionary Biology UnitSouth Australian MuseumNorth Terrace, AdelaideAustralia
  6. 6.Department of FisheriesWestern Australia Fisheries and Marine Research LaboratoriesNorth BeachAustralia
  7. 7.School of Animal BiologyThe University of Western AustraliaCrawleyAustralia

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