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Isolation and characterization of 13 polymorphic microsatellite loci for the smooth Cherax cainii and hairy marron C. tenuimanus (Decapoda: Parastacidae)

  • Microsatellite Letters
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Abstract

Numbers of the critically endangered hairy marron Cherex tenuimanus are declining rapidly in its natural habitat, mainly due to competition with smooth marron C. cainii. To determine whether genetic introgression is occurring between the two species we isolated 13 new polymorphic microsatellite markers from C. cainii using 454 shotgun sequencing. The loci were screened for variation in 20 individuals of C. cainii and eight individuals of C. tenuimanus. There was no evidence of linkage disequilibrium between pairs of loci and only one locus deviated significantly from Hardy–Weinberg equilibrium. The number of alleles per locus ranged from two to 11 across both species.

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References

  • Austin CM, Ryan SG (2002) Allozyme evidence for a new species of freshwater crayfish of the genus Cherax Erichson (Decapoda: Parastacidae) from the south-west of Western Australia. Invertebr Syst 16:357–367

    Article  Google Scholar 

  • Gardner MG, Fitch AJ, Bertozzi T, Lowe AJ (2011) Rise of the machines—recommendations for ecologists when using next generation sequencing for microsatellite development. Mol Ecol Resour 11:1093–1101

    Article  PubMed  Google Scholar 

  • Kalinowski S, Taper M, Marshall T (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1006–1099

    Article  Google Scholar 

  • Meglecz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin J-F (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404

    Article  CAS  PubMed  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

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Acknowledgments

We acknowledge the assistance and support of Alison Fitch from Flinders University. This project was funded by the Western Australian Department of Fisheries.

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Authors and Affiliations

  1. Centre for Evolutionary Biology, School of Animal Biology, The University of Western Australia, Crawley, WA, 6009, Australia

    W. Jason Kennington, Clodagh Guildea, Sherralee S. Lukehurst & P. Joana Dias

  2. Helix Molecular Solutions, PO Box 155, Leederville, WA, 6903, Australia

    Yvette Hitchen

  3. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA, 5001, Australia

    Michael G. Gardner

  4. Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide, SA, 5005, Australia

    Michael G. Gardner

  5. Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA, 5000, Australia

    Michael G. Gardner

  6. Department of Fisheries, Western Australia Fisheries and Marine Research Laboratories, PO Box 20, North Beach, WA, 6920, Australia

    Rodney Duffy, P. Joana Dias & Michael Snow

  7. School of Animal Biology, The University of Western Australia, Crawley, WA, 6009, Australia

    Jason M. Ledger

Authors
  1. W. Jason Kennington
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  2. Clodagh Guildea
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  3. Sherralee S. Lukehurst
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  4. Yvette Hitchen
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  5. Michael G. Gardner
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  6. Rodney Duffy
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  7. P. Joana Dias
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  8. Jason M. Ledger
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  9. Michael Snow
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Corresponding author

Correspondence to W. Jason Kennington.

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Jason Kennington, W., Guildea, C., Lukehurst, S.S. et al. Isolation and characterization of 13 polymorphic microsatellite loci for the smooth Cherax cainii and hairy marron C. tenuimanus (Decapoda: Parastacidae). Conservation Genet Resour 6, 337–339 (2014). https://doi.org/10.1007/s12686-013-0088-1

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  • DOI: https://doi.org/10.1007/s12686-013-0088-1

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