Advertisement

Conservation Genetics Resources

, Volume 6, Issue 2, pp 327–328 | Cite as

Development and characterisation of 12 microsatellite markers for the New Zealand endemic scallop Pecten novaezelandiae

  • Catarina N. S. Silva
  • Jonathan P. A. Gardner
Microsatellite Letters

Abstract

The endemic scallop Pecten novaezelandiae supports important fisheries in New Zealand. However, despite the ecological and economic importance of this species, limited information is known about genetic stock structure and genetic connectivity among populations. Using 454 sequencing we developed 12 polymorphic microsatellite markers for this scallop. The number of alleles per locus ranged from 6 to 37 and no significant linkage disequilibrium was detected between locus pairs. Three loci (Pnova_01, Pnova_24 and Pnova_27) showed significant deviations from Hardy–Weinberg equilibrium, most likely because of null alleles. The new markers are currently being used to assess the levels of genetic variability among populations to contribute to conservation and management of this highly exploited mollusc.

Keywords

Scallop Pecten novaezelandiae Bivalvia Pectinidae Microsatellites New Zealand 

Notes

Acknowledgments

The authors would like to thank JR Williams and D McNaughtan for the sample collection and HB Constable, D Hannan and C Zeng for assisting with the experiment. Financial support was provided by the New Zealand Ministry of Primary Industries (ZBD2009-10) and Victoria University of Wellington Doctoral Scholarship.

References

  1. Holleley CE, Geerts PG (2009) Multiplex manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR. Biotechniques 46:511–517PubMedCrossRefGoogle Scholar
  2. Marín A, Fujimoto T, Arai K (2012) Isolation and characterization of 12 microsatellite loci in the Peruvian scallop Argopecten purpuratus and cross-species amplification in other scallop species (family Pectinidae). Conserv Genet Resour 4:179–182CrossRefGoogle Scholar
  3. Meglécz E, Costedoat C, Dubut V et al (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404PubMedCrossRefGoogle Scholar
  4. Rousset F (2008) genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8:103–106PubMedCrossRefGoogle Scholar
  5. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-Checker: software for Identifying and Correcting Genotyping Errors in Microsatellite Data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  6. Williams JR, Parkinson DM (2010) Biomass survey and stock assessment for the Coromandel scallop fishery, 2010. Ministry of Fisheries, WellingtonGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Catarina N. S. Silva
    • 1
  • Jonathan P. A. Gardner
    • 1
  1. 1.School of Biological SciencesVictoria University of WellingtonWellingtonNew Zealand

Personalised recommendations