Development and characterisation of 12 microsatellite markers for the New Zealand endemic scallop Pecten novaezelandiae
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The endemic scallop Pecten novaezelandiae supports important fisheries in New Zealand. However, despite the ecological and economic importance of this species, limited information is known about genetic stock structure and genetic connectivity among populations. Using 454 sequencing we developed 12 polymorphic microsatellite markers for this scallop. The number of alleles per locus ranged from 6 to 37 and no significant linkage disequilibrium was detected between locus pairs. Three loci (Pnova_01, Pnova_24 and Pnova_27) showed significant deviations from Hardy–Weinberg equilibrium, most likely because of null alleles. The new markers are currently being used to assess the levels of genetic variability among populations to contribute to conservation and management of this highly exploited mollusc.
KeywordsScallop Pecten novaezelandiae Bivalvia Pectinidae Microsatellites New Zealand
The authors would like to thank JR Williams and D McNaughtan for the sample collection and HB Constable, D Hannan and C Zeng for assisting with the experiment. Financial support was provided by the New Zealand Ministry of Primary Industries (ZBD2009-10) and Victoria University of Wellington Doctoral Scholarship.
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