Testing mitochondrial markers for noninvasive genetic species identification in European mammals
- 366 Downloads
While DNA-barcoding routines have largely facilitated molecular identification of animal species, there is no common strategy for species identifications based on noninvasively collected samples. We compared the performance of six commonly used mitochondrial fragments for identification success of low-quality hair and scat samples from 15 European mammals. The standard cytochrome oxidase subunit 1 barcoding fragment performed worst in the test, with only three successful species identifications out of 32 samples. In contrast, three short fragments (16S and 12S rDNA, cytochrome b) led to reliable species identifications for >80 % of all tested samples. Success rates also differed among feeding types.
KeywordsNoninvasive sampling Species discrimination Mammalia Genetic monitoring Wildlife forensics
Several wildlife parks provided kind sampling assistance. Sequence reaction were performed in the BiK-F Laboratory Centre, Frankfurt. This project was funded by the Leibniz-Association in the context of the “Joint Initiative for Research and Innovation” (SAW-Pakt).
- Cheney LC (1995) An assessment of genetic variation within and between sea otter (Enhydra lutris) populations off Alaska and California. Master′s Thesis, San Jose State UniversityGoogle Scholar
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotech 3(5):294–299Google Scholar
- Karamanlidis AA, Straka M, Drosopoulou E, Hernando MD, Kocijan I, Paule L, Scouras Z (2012) Genetic diversity, structure, and size of an endangered brown bear population threatened by highway construction in the Pindos Mountains, Greece. Eur J Wildl Res 58(3):511–522. doi: 10.1007/s10344-011-0598-7 CrossRefGoogle Scholar