Skip to main content

Isolation and characterization of 15 microsatellite loci in the specialist butterfly Boloria eunomia


Boloria eunomia is a boreo-montane butterfly species suffering from habitat loss and isolation in the relictual part of its distribution range. Small populations persist in habitats scattered on plateaux or low mountains in western, central and southern Europe. Quantifying gene flow within and between these remnant populations is thus a crucial point to properly delineate metapopulations, to understand their dynamics and hence to design appropriate conservation plans for this butterfly species. We developed primers for the amplification of 15 microsatellites loci for B. eunomia. Thirteen loci were grouped in 2 multiplexes and amplified in 50 individuals from 5 populations to validate their use in population genetics. Gene diversity was on average 0.63 across populations. Null alleles and recurrent Hardy–Weinberg disequilibrium were detected in 7 loci. However, Fst estimates after correction for the presence of null alleles were highly correlated (0.91) to Fst estimates without correction. The loci developed here are thus usable for fine scale population genetic analyses and represent a very useful tool to quantify gene flow within and between metapopulations of B. eunomia.

This is a preview of subscription content, access via your institution.


  • Baguette M, Clobert J, Schtickzelle N (2011) Metapopulation dynamics of the bog fritillary butterfly. Experimental changes in habitat quality induced negative density-dependent dispersal. Ecography 34:170–176

    Article  Google Scholar 

  • Hamilton MB, Pincus EL, Di Fiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507

    CAS  PubMed  Google Scholar 

  • Nève G, Barascud B, Descimon H, Baguette M (2008) Gene flow rises with habitat fragmentation in the bog fritillary butterfly (Lepidoptera: Nymphalidae). BMC Evol Biol 17(8):84

    Article  Google Scholar 

  • Sinama M, Dubut V, Costdoat C, Gilles A, Junker M, Malausa T, Martin JF, Pech N, Schmitt T, Zimmermann M, Meglecz E (2011) Challenges of microsatellite development in Lepidoptera: Euphydryas aurinia (Nymphalidae) as a case study. Eur J Entomol 108:261–266

    Article  Google Scholar 

  • Vandewoestijne S, Baguette M (2004) Genetic population of the vulnerable bog fritillary butterfly. Hereditas 3:199–206

    Google Scholar 

Download references


This study was supported by the ANR (French National Research Agency) through the FP6 Biodiversa ERANET TenLamas project. D.L., M.H., V.M.S. and M.B. are part of the Laboratoire d’Excellence TULIP (ANR-10-LABX-41).

Author information

Authors and Affiliations


Corresponding author

Correspondence to Delphine Legrand.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Legrand, D., Chaput-Bardy, A., Turlure, C. et al. Isolation and characterization of 15 microsatellite loci in the specialist butterfly Boloria eunomia . Conservation Genet Resour 6, 223–227 (2014).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


  • Null Allele
  • Polymorphic Information Content
  • Butterfly Species
  • False Discovery Rate Correction
  • Estimate Gene Flow