Conservation Genetics Resources

, Volume 6, Issue 1, pp 185–187 | Cite as

Isolation and characterization of fifteen microsatellite loci in Leopard grouper (Mycteroperca rosacea) via 454 pyrosequencing

  • Alexis M. Jackson
  • Adrian Munguía-Vega
  • Avian S. Lain
  • Simon A. Stokes
  • Aneese J. Williams
  • Giacomo Bernardi
Microsatellite Letters

Abstract

Leopard grouper (Mycteroperca rosacea) are distributed from Bahía Magdalena, through the Gulf of California and as far south as Jalisco, Mexico. They are listed as Vulnerable on the IUCN Red List due to significant fishing-induced declines over the past decade. Microsatellite loci were isolated by constructing a shotgun genomic library and sequencing using 454 XL + titanium chemistry. We characterized 15 polymorphic loci in 120 samples from three geographic locations. Genotyping yielded 15–60 alleles per locus with observed levels of heterozygosity ranging from 0.575 to 0.975.

Keywords

Leopard grouper Mycteroperca rosacea Microsatellites Fisheries management Conservation 

Supplementary material

12686_2013_44_MOESM1_ESM.doc (59 kb)
Supplementary material 1 (DOC 59 kb)

References

  1. Craig MT, Sadovy Y (2008) Mycteroperca rosacea. IUCN 2010. IUCN Red List of Threatened Species. Version 2010.4. IUCNGoogle Scholar
  2. Jackson AM, Semmens BX, Bernardi G (2012) Characterization and cross-species amplification of microsatellite markers in Nassau grouper (Epinephelus striatus). Mol Ecol Resour 12(5):972–974PubMedCrossRefGoogle Scholar
  3. Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin J-F (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26(3):403–404PubMedCrossRefGoogle Scholar
  4. Sambrook J, Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual second edition Vols. 1, 2, and 3. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar
  5. Thomson DA, Findley LT, Kerstitch AN (2000) Reef fishes of the sea of Cortez. University of Texas Press, AustinGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Alexis M. Jackson
    • 1
  • Adrian Munguía-Vega
    • 2
    • 3
  • Avian S. Lain
    • 1
    • 4
  • Simon A. Stokes
    • 5
  • Aneese J. Williams
    • 1
    • 6
  • Giacomo Bernardi
    • 1
  1. 1.Department of Ecology and Evolutionary BiologyUniversity of California Santa CruzSanta CruzUSA
  2. 2.School of Natural Resources and the EnvironmentUniversity of ArizonaTucsonUSA
  3. 3.Comunidad y Biodiversidad A.C.GuaymasMexico
  4. 4.Department of Chemical EngineeringHampton UniversityHamptonUSA
  5. 5.Department of Molecular, Cellular and Developmental BiologyUniversity of California Santa CruzSanta CruzUSA
  6. 6.Department of Environmental and Marine ScienceHampton UniversityHamptonUSA

Personalised recommendations