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Development and characterization of microsatellite markers for giant sequoia, Sequoiadendron giganteum (Cupressaceae)

  • Microsatellite Letters
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Abstract

We describe eleven polymorphic microsatellite markers for giant sequoia [Sequoiadendron giganteum (Lindl.) J. Buchh.], the world’s largest tree. Size-selected genomic DNA was enriched for short sequence repeats (SSR) and the resulting SSR library was analyzed on a Roche 454 platform. DNA samples from seedlings grown in a common garden (n = 359), representing a gene pool from twenty-three groves, were examined for SSR polymorphisms. The total observed alleles per locus ranged from three to twenty-five. Samples from the cabin creek grove (n = 21) exhibit observed heterozygosities ranging from 0.19 to 1.0, with one loci displaying significant deviation from Hardy–Weinberg equilibrium. Within this grove, no loci showed significant linkage disequilibrium. These microsatellite markers are important for assessing and conserving genetic diversity in this rare conifer.

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Authors and Affiliations

  1. University of California at Berkeley, Berkeley, CA, USA

    Rainbow DeSilva & Richard Dodd

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  1. Rainbow DeSilva
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  2. Richard Dodd
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Correspondence to Rainbow DeSilva.

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DeSilva, R., Dodd, R. Development and characterization of microsatellite markers for giant sequoia, Sequoiadendron giganteum (Cupressaceae). Conservation Genet Resour 6, 173–174 (2014). https://doi.org/10.1007/s12686-013-0039-x

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  • DOI: https://doi.org/10.1007/s12686-013-0039-x

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