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Isolation and characterization of microsatellites in the endoparasitic ichneumonid wasp carrying a polydnavirus Hyposoter didymator

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Abstract

The wasp Hyposoter didymator (Hymenoptera, Ichneumonidae) parasitizes several agricultural pest moths and could therefore be used in biological control. The 454 FLX Titanium pyrosequencing technology was used to define two distinct sets of multiplex combining 14 polymorphic microsatellite loci: 10 (referred to as HD) located within the genome of H. didymator and 4 (referred to as HdIV) located within the Ichnovirus genome which is integrated into the wasp genome. Genotyping of two populations collected in France on Helicoverpa armigera revealed that most of the loci are independent and at Hardy–Weinberg equilibrium.

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Acknowledgments

The Titanium pyrosequencing was funded by the grant AIP BioRessources EcoMicro from Inra. Genotyping was part of a project funded by the “Département Santé des Plantes et Environnement” from Inra. Data were (partly) produced through molecular genetic analysis technical facilities of the labex “Centre Méditerranéen de l’Environnement et de la Biodiversité”.

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Authors and Affiliations

  1. CBGP, UMR INRA-IRD-CIRAD-Montpellier SupAgro, Campus International de Baillarguet, CS 30016, 34988, Montferrier-sur-Lez Cedex, France

    Philippe Audiot & Denis Bourguet

  2. DGIMI, INRA-Université Montpellier 2, Place Eugène Bataillon, CC101, 34095, Montpellier Cedex, France

    Véronique Jouan, Marie Frayssinet & Anne-Nathalie Volkoff

Authors
  1. Philippe Audiot
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  2. Véronique Jouan
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  3. Marie Frayssinet
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  4. Anne-Nathalie Volkoff
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  5. Denis Bourguet
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Correspondence to Philippe Audiot.

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Audiot, P., Jouan, V., Frayssinet, M. et al. Isolation and characterization of microsatellites in the endoparasitic ichneumonid wasp carrying a polydnavirus Hyposoter didymator . Conservation Genet Resour 6, 21–23 (2014). https://doi.org/10.1007/s12686-013-0036-0

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  • DOI: https://doi.org/10.1007/s12686-013-0036-0

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