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Isolation and characterisation of novel microsatellite markers from Pagrus auratus and cross amplification in Acanthopagrus butcheri

  • Microsatellite Letters
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Abstract

We describe the isolation and characterisation of microsatellite loci for Pagrus auratus and cross amplification in another sparid, Acanthopagrus butcheri. Of 35 loci tested, 12 appear to be appropriate for population genetic studies of P. auratus, which are essential to inform management responses to evidence of overexploitation of this species. Three were ideal for A. butcheri and, when combined with published loci, can be used to assess the genetic implications of restocking depleted populations of this species.

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Acknowledgments

We thank Frances Brigg for help with the fragment analysis. We also thank Trevor Price, Greg Jenkins, David Fairclough, Gary Jackson and Jeff Norriss for samples and Ertug Sezmis for DNA extractions for the library preparation. This research was supported by the Western Australian Marine Science Institution and Murdoch University.

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Authors and Affiliations

  1. Centre for Fish and Fisheries Research Murdoch University, Perth, WA, 6150, Australia

    Michelle J. Gardner, Jennifer A. Chaplin & Nicole M. Phillips

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  1. Michelle J. Gardner
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  2. Jennifer A. Chaplin
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  3. Nicole M. Phillips
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Correspondence to Michelle J. Gardner.

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Gardner, M.J., Chaplin, J.A. & Phillips, N.M. Isolation and characterisation of novel microsatellite markers from Pagrus auratus and cross amplification in Acanthopagrus butcheri . Conservation Genet Resour 6, 155–157 (2014). https://doi.org/10.1007/s12686-013-0031-5

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  • DOI: https://doi.org/10.1007/s12686-013-0031-5

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