Abstract
Microsatellite loci (26 in total) were isolated for the first time for the entocommensal nemertean Malacobdella arrokeana, using 454 GS-FLX Titanium pyrosequencing. We developed conditions for amplifying these markers in 8 multiplex and 5 individual reactions. One to 14 alleles were detected per locus across 25 samples analyzed from San Matías Gulf, Patagonia (Argentina). For the 25 polymorphic loci, observed and expected heterozygosities ranged from 0.125 to 0.920 and 0.119 to 0.890, respectively; five loci deviated from Hardy–Weinberg equilibrium. Limited distribution and host specificity of M. arrokeana, which only inhabits Panopea abbreviata, have endangered this nemertean given the unregulated commercial exploitation of its host. These useful markers provide data for future conservation strategies. Cross-amplifications were also tested in 34 nemerteans species, representing the major lineages in this phylum. In total, 18 of the 26 loci (from one to 11 per species) gave clear allelic profiles.
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References
Alfaya JEF, Bigatti G, Machordom A (2013) Mitochondrial and nuclear markers reveal a lack of genetic structure in the entocommensal nemertean Malacobdella arrokeana in the Patagonian gulfs. Helgoland Mar Res 67:407–412
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
Ivanov VA, Bigatti G, Penchaszadeh PE, Norenburg JL (2002) Malacobdella arrokeana (Nemertea: Bdellonemertea), a new species of nemertean from the Southwestern Atlantic Ocean entocommensal in Panopea abbreviata (Bivalvia, Heterodonta, Hiatellidae) in Argentina. Proc Biol Soc Wash 115:359–367
López-Márquez V, Alfaya JEF, García-Jiménez R, Bigatti G, Machordom A (2013) Isolation and characterisation of microsatellite loci for the southern geoduck Panopea abbreviata (Valenciennes, 1839) through 454 pyrosequencing. In: Ahanchédé A, Alfaya JEF, Andersen LW, et al. Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2012–30 September 2012. Mol Ecol Resour 13:158–159
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICROCHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
We would like to thank Steve Bogdanowicz from the Cornell EGCF for his technical assistance during microsatellite isolation. Melinda Modrell revised the English. This research was funded by the Spanish Ministry of Foreign Affairs through AECID grants A/023484/09 and A/032441/10.
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José E. F. Alfaya and Violeta López-Márquez contributed equally to this work.
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Alfaya, J.E.F., López-Márquez, V., García-Jiménez, R. et al. Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species. Conservation Genet Resour 6, 147–150 (2014). https://doi.org/10.1007/s12686-013-0029-z
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DOI: https://doi.org/10.1007/s12686-013-0029-z