Development, characterisation and cross-species amplification of 16 novel microsatellite markers for the endangered Black-throated Finch (Poephila cincta) in Australia
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The Black-throated Finch (Southern) (Poephila cincta cincta) is threatened by the substantial landscape changes in northern Australia. We developed 16 polymorphic microsatellite markers using 454-shotgun whole-genome sequencing technology. We identified an average of 4.7 alleles per locus based on 63 wild caught individuals from Townsville, Queensland. Thirteen and 9 markers were also successfully cross-amplified in two confamilial species, the Double-barred Finch (Taeniopygia bichenovii) and the chestnut-breasted Mannikin (Lonchura castaneothorax) with 11 and 5 were polymorphic, respectively. These markers will help understand the population genetic structure of the endangered Black-throated Finch and determine genetic consequences of landscape changes for the species.
KeywordsBlack-throated Finch Poephila cincta Microsatellite Conservation genetics Next generation sequencing
We especially thank Dr Tony Grice and Dr James Moloney for their valuable help and comments throughout this work. Juliana Rechetelo, Dr April Reside, Troy Countryman, Matthew McIntosh and other volunteers assisted with the fieldwork. We are also grateful to Blanche D’Anastasi, Erica Todd, Georgia McDougall and Alison Fitch for their general laboratory assistance and advice. The development of these primers was conducted within the Molecular Ecology & Evolutionary Laboratory (James Cook University) and funded by the Black-throated Finch Trust and School of Earth & Environmental Sciences at James Cook University.
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