Development of microsatellite markers for Virola officinalis Warb. (Myristicaceae), an endemic tree of the Brazilian Atlantic rain forest by next generation sequencing technology
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We report the development and characterization of microsatellite markers for Virola officinalis, an endemic Brazilian Atlantic rain forest tree and important food resource for local wildlife. Seventy-two individuals were collected to characterize the 21 primers developed by next generation sequencing. Of the 21 primers studied, 15 were polymorphic. On average, the expected heterozygosity per population ranged from 0.64 to 0.73. The combined probability of exclusion was higher than 0.999, indicating that the microsatellite markers developed are suitable for genetic structure investigations and paternity testing. Our results will contribute to the design of efficient conservation strategies not only for this species, but for the Atlantic rain forest as a whole.
KeywordsNGS Primer Conservation genetics Endangered species
This is the publication # 001 of the Rede SisbiotaUna. The authors thank Dr. Deborah Maria de Faria, Dr. Cassio van Berg, Dr. Maria Cristina López Roberts, Daniele França, Horlei Ribeiro, and all members of the Molecular Markers Laboratory, UESC for technical assistance This work was supported by the SISBIOTA Project (563216/2010-7), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES).
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