Conservation Genetics Resources

, Volume 6, Issue 1, pp 83–86 | Cite as

Development of microsatellite markers for Virola officinalis Warb. (Myristicaceae), an endemic tree of the Brazilian Atlantic rain forest by next generation sequencing technology

  • Flora Bittencourt
  • Roberto Tarazi
  • Fernanda A. GaiottoEmail author
Microsatellite Letters


We report the development and characterization of microsatellite markers for Virola officinalis, an endemic Brazilian Atlantic rain forest tree and important food resource for local wildlife. Seventy-two individuals were collected to characterize the 21 primers developed by next generation sequencing. Of the 21 primers studied, 15 were polymorphic. On average, the expected heterozygosity per population ranged from 0.64 to 0.73. The combined probability of exclusion was higher than 0.999, indicating that the microsatellite markers developed are suitable for genetic structure investigations and paternity testing. Our results will contribute to the design of efficient conservation strategies not only for this species, but for the Atlantic rain forest as a whole.


NGS Primer Conservation genetics Endangered species 



This is the publication # 001 of the Rede SisbiotaUna. The authors thank Dr. Deborah Maria de Faria, Dr. Cassio van Berg, Dr. Maria Cristina López Roberts, Daniele França, Horlei Ribeiro, and all members of the Molecular Markers Laboratory, UESC for technical assistance This work was supported by the SISBIOTA Project (563216/2010-7), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES).


  1. Ekblom R, Galindo J (2011) Applications of next generation sequencing in molecular ecology of non-model organisms. Heredity 107:1–15. doi: 10.1038/hdy.2010.152 PubMedCrossRefGoogle Scholar
  2. Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9. 3). Lausanne University, Switzerland. Accessed 13 April 2013
  3. Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106. doi: 10.1111/j.1365-294x.2007.03089.x PubMedCrossRefGoogle Scholar
  4. Martini AMZ, Fiaschi P, Amorim AM, Paixão JL (2007) A hot-point within a hot-spot: a high diversity site in Brazil’s Atlantic forest. Biodivers Conserv 16(11):3111–3128. doi: 10.1007/s10531-007-9166-6 CrossRefGoogle Scholar
  5. Rodrigues WA (1980) Revisão Taxonômica das espécies de Virola Aublet (Myristicaceae) do Brasil. Tese. University of Campinas/UNICAMP. vol. I e IIGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Flora Bittencourt
    • 1
  • Roberto Tarazi
    • 1
  • Fernanda A. Gaiotto
    • 1
    Email author
  1. 1.Laboratory of Molecular Markers, Center of Biotechnology and Genetics (CBG)State University of Santa Cruz (UESC)IlhéusBrazil

Personalised recommendations