New microsatellite loci for the threatened eastern hog-nosed snake (Heterodon platirhinos) in Ontario, Canada

Abstract

The eastern hog-nosed snake (Heterodon platirhinos) is a federally listed species of conservation concern in Canada. Microsatellite markers are desired for research on the spatial structure, degree of isolation and neutral genetic status of the two regional isolated populations in the province of Ontario. We developed 14 microsatellite loci and genotyped 27 individuals from one of the two regional populations. The total number of alleles and the level of expected heterozygosity ranged from 4 to 8 and from 0.35 to 0.84, respectively. These microsatellite loci will be useful for assessing neutral genetic diversity and demarcating functionally independent groups for effective management practices.

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Acknowledgments

We thank Zhengxin Sun for assistance in genotyping and Jake Rouse for sample collections. Permission to collect samples was Granted by the Ontario Ministry of Natural Resources, Ontario Parks, and Parks Canada. Funding was provided by the Species at Risk Research Fund for Ontario and the Natural Sciences and Engineering Research Council of Canada (NSERC Discovery Grant to SCL and CGS-M to ATJX).

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Correspondence to Stephen C. Lougheed.

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Xuereb, A.T.J., Austin, J.D. & Lougheed, S.C. New microsatellite loci for the threatened eastern hog-nosed snake (Heterodon platirhinos) in Ontario, Canada. Conservation Genet Resour 6, 69–71 (2014). https://doi.org/10.1007/s12686-013-0007-5

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Keywords

  • Microsatellites
  • Eastern hog-nosed snake
  • Heterodon platirhinos
  • 454 Sequencing