Novel microsatellite loci for winter flounder (Pseudopleuronectus americanus)

Abstract

Since the 1980s, heavy exploitation has led to severe population declines in winter flounder, Pseudopleuronectes americanus, a demersal flatfish inhabiting the inshore bays and estuaries of the Northwest Atlantic. Recent studies using a limited number of non-specific microsatellite loci have demonstrated fine-scale population structure. Here, we present 21 novel species-specific microsatellite loci to enhance studies of genetic diversity, population differentiation, effective population size and mating behavior of this important commercial species.

This is a preview of subscription content, log in to check access.

References

  1. Buckley J (1989) Species profiles: life histories and environmental requirements of coastal fishes and invertebrates (North Atlantic)—winter flounder. U.S. Fish Wildl Serv Biol Rep 82(11.87): U.S. Army Corps of Engineers, TR EL-82-84

  2. Crivello JF, Danila DJ, Lorda E, Keser M, Roseman EF (2004) The genetic stock structure of larval and juvenile winter flounder larvae in Connecticut waters of eastern Long Island Sound and estimations of larval entrainment. J Fish Biol 65:62–76

    Article  Google Scholar 

  3. DeCelles GR, Cadrin SX (2007) An interdisciplinary assessment of winter flounder stock structure. ICES CM 18

  4. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Method Enzymol 395:202–222

    Article  CAS  Google Scholar 

  5. Goudet J (1995) Fstat version 1.2: a computer program to calcluate Fstatistics. J Hered 86(6):485–486

    Google Scholar 

  6. Hendrickson LNP, Terceiro M (2006) Status of fishery resources off the Northeastern US—Winter Flounder. NEFSC—Resource Evaluation and Assessment Division

  7. Hoarau G, Boon E, Jongma DN, Ferber S, Palsson J, Van der Veer HK, Rijnsdorp AD, Stam WT, Olsen JL (2004) Low effective population size and evidence for inbreeding in an overexploited flatfish, plaice (Pleuronectes platessa L.). Proc R Soc B 272(1562):497–503

    Article  Google Scholar 

  8. McClelland G, Melendy J, Osborne J, Reid D, Douglas S (2005) Use of parasite and genetic markers in delineating populations of winter flounder from the central and south-west Scotian Shelf and north-east Gulf of Maine. J Fish Biol 66(4):1082–1100

    Article  Google Scholar 

  9. McCracken FD (1963) Seasonal movements of the winter flounder, Pseudopleuronectes americanus (Wahlbaum), on the Atlantic Coast. J Fish Res Board Can 20(2):551–586

    Article  Google Scholar 

  10. Northeast Fisheries Science Center (2011) 52nd Northeast Regional Stock Assessment Workshop (52nd SAW) Assessment Report. NEFSC Ref Doc 11-17; 962 p. Available from: National Marine Fisheries Service, 166 Water Street, Woods Hole, MA 02543-1026, or online at http://nefsc.noaa.gov/publications/

  11. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. Heredity 86:2480249

    Google Scholar 

  12. Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linus. Mol Ecol Res 8:103–106

    Article  Google Scholar 

  13. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    PubMed  Article  CAS  Google Scholar 

  14. van Oosterhout C, Hutchinson WF, Willis DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Shannon J. O’Leary.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

O’Leary, S.J., Feldheim, K.A. & Chapman, D.D. Novel microsatellite loci for winter flounder (Pseudopleuronectus americanus). Conservation Genet Resour 5, 569–572 (2013). https://doi.org/10.1007/s12686-012-9854-8

Download citation

Keywords

  • Flatfish
  • Population genetics
  • Population structure
  • Genetic diversity