After identifying 114 microsatellite loci from Choristoneura fumiferana expressed sequence tags, 87 loci were assayed in a panel of 11 wild-caught individuals, giving 29 polymorphic loci. Further analysis of 20 of these loci on 31 individuals collected from a single population in northern Minnesota identified 14 in Hardy–Weinberg equilibrium.
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We thank P. Stothard for assistance in post-processing and analysis of ESTs, and P. Beldade and A. Long for the use of their SSR-mining Perl script. This study was funded by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada to F. Sperling, a Genomics Research and Development Initiative Grant (NRCAN) to D. Doucet, and a United States Department of Agriculture, Cooperative State Research Education and Extension Service, National Research Initiative Grant (WISR-2005-02815) to Brian Sturtevant.
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Online Resource 1: Characteristics of 87 EST-SSR loci tested on a validation panel of 11 individuals from Alberta. (XLS 146 kb)
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Brunet, B.M.T., Doucet, D., Sturtevant, B.R. et al. Characterization of EST-based SSR loci in the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae). Conservation Genet Resour 5, 541–544 (2013). https://doi.org/10.1007/s12686-012-9847-7
- Spruce budworm
- Expressed sequence tag
- Simple sequence repeat
- Choristoneura fumiferana