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Characterization of EST-based SSR loci in the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae)

Conservation Genetics Resources volume 5, pages 541–544 (2013)Cite this article

Abstract

After identifying 114 microsatellite loci from Choristoneura fumiferana expressed sequence tags, 87 loci were assayed in a panel of 11 wild-caught individuals, giving 29 polymorphic loci. Further analysis of 20 of these loci on 31 individuals collected from a single population in northern Minnesota identified 14 in Hardy–Weinberg equilibrium.

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Acknowledgments

We thank P. Stothard for assistance in post-processing and analysis of ESTs, and P. Beldade and A. Long for the use of their SSR-mining Perl script. This study was funded by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada to F. Sperling, a Genomics Research and Development Initiative Grant (NRCAN) to D. Doucet, and a United States Department of Agriculture, Cooperative State Research Education and Extension Service, National Research Initiative Grant (WISR-2005-02815) to Brian Sturtevant.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Alberta, 11455 Saskatchewan Drive, Edmonton, AB, T6G 2E9, Canada

    B. M. T. Brunet & F. A. H. Sperling

  2. Natural Resources Canada, Great Lakes Forestry Centre, 1219 Queen St. East, Sault Ste. Marie, ON, P6A 2E5, Canada

    D. Doucet

  3. USDA Forest Service, Northcentral Research Station, 5985 Highway K, Rhinelander, WI, 54501, USA

    B. R. Sturtevant

Authors
  1. B. M. T. Brunet
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  2. D. Doucet
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  3. B. R. Sturtevant
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  4. F. A. H. Sperling
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Corresponding author

Correspondence to B. M. T. Brunet.

Electronic supplementary material

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12686_2012_9847_MOESM1_ESM.xls

Online Resource 1: Characteristics of 87 EST-SSR loci tested on a validation panel of 11 individuals from Alberta. (XLS 146 kb)

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Brunet, B.M.T., Doucet, D., Sturtevant, B.R. et al. Characterization of EST-based SSR loci in the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae). Conservation Genet Resour 5, 541–544 (2013). https://doi.org/10.1007/s12686-012-9847-7

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  • DOI: https://doi.org/10.1007/s12686-012-9847-7

Keywords

  • Spruce budworm
  • Expressed sequence tag
  • Microsatellite
  • Simple sequence repeat
  • Choristoneura fumiferana
  • Tortricidae