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Conservation Genetics Resources

, Volume 5, Issue 2, pp 529–531 | Cite as

Isolation and characterisation of ten microsatellite markers for the tetraploid Stypandra glauca R.Br. (Hemerocallidaceae) identified using next generation sequencing

  • S.-L. Tapper
  • M. Byrne
  • M. G. Gardner
Technical Note

Abstract

Ten polymorphic microsatellite loci were developed for the tetraploid plant species Stypandra glauca which is common on granite outcrops in Western Australia. Amplification and genotyping trials were conducted on 48 individuals from two sampling localities. All 10 loci revealed multi-banding patterns with up to 4 bands visible in individuals consistent with tetraploidy. The number of alleles per locus ranged from 6 to 32 (mean = 12.5). The proportion of observed heterozygotes at each locus ranged from 0.32 to 0.98 (mean = 0.73). These polymorphic microsatellite markers will facilitate further analysis of population genetic structure and connectivity in Stypandra glauca.

Keywords

Granite outcrop Refugia Western Australia Genetic diversity 

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Science Division, Department of Environment and ConservationBentley Delivery CentreKensingtonAustralia
  2. 2.School of Biological SciencesFlinders UniversityAdelaideAustralia
  3. 3.Evolutionary Biology UnitSouth Australian MuseumAdelaideAustralia

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