Conservation Genetics Resources

, Volume 5, Issue 2, pp 519–524

Large-scale isolation of Eastern spiny mouse Acomys dimidiatus microsatellite loci through GS-FLX 454 titanium sequencing

  • Sanad Alfadala
  • Deborah A. Dawson
  • Gavin J. Horsburgh
  • Jerzy M. Behnke
  • Anna Bajer
  • Eman M. E. Mohallal
  • Samy Zalat
  • Jon Slate
Technical Note
  • 294 Downloads

Abstract

We isolated and characterized Eastern spiny mouse, Acomys dimidiatus microsatellite loci. A microsatellite-enriched library was created and A. dimidiatus fragments sequenced using 454 sequencing. In total, 1,221 primer-designable microsatellite sequences were identified. We designed primer sets for 20 loci. Loci were characterized in A. dimidiatus individuals from a semi-isolated desert wadi (valley) in St. Katherine Protectorate, Egypt. After initial trails, 18 microsatellite loci were genotyped in 67 mice. The number of alleles displayed in the 18 markers ranged from three to nine (mean = 6) with mean expected and observed heterozygosities of 0.63 and 0.65, respectively. All 18 selected loci were in Hardy–Weinberg equilibrium (P > 0.01). These markers will be used to investigate the fine-scale spatial patterns of genetic diversity and divergence of A. dimidiatus populations. The isolated loci are of potential utility in other murines, including 260 threatened species.

Keywords

Eastern spiny mouse Pyrosequencing Microsatellite markers Muridae Rodent Simple sequence repeats (SSRs) 

Supplementary material

12686_2012_9842_MOESM1_ESM.xls (4 mb)
Supplementary material 1 (XLS 4046 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Sanad Alfadala
    • 1
  • Deborah A. Dawson
    • 1
  • Gavin J. Horsburgh
    • 1
  • Jerzy M. Behnke
    • 2
  • Anna Bajer
    • 3
  • Eman M. E. Mohallal
    • 4
  • Samy Zalat
    • 5
    • 6
  • Jon Slate
    • 1
  1. 1.Department of Animal and Plant SciencesUniversity of SheffieldSheffieldUK
  2. 2.School of BiologyUniversity of NottinghamNottinghamUK
  3. 3.Department of Parasitology, Institute of ZoologyUniversity of WarsawWarsawPoland
  4. 4.Desert Research CentreCairoEgypt
  5. 5.Department of ZoologySuez Canal UniversityIsmailiaEgypt
  6. 6.Faculty of Arts and SciencesTaibah University El-Ula BranchEl-UlaSaudi Arabia

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