Characterization of 13 polymorphic microsatellite loci in Rimicaris hybisae, a shrimp from deep-sea hydrothermal vents
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Rimicaris hybisae is a deep-sea alvinocaridid shrimp found at hydrothermal vents along the Mid-Cayman Spreading Center. Eleven selectively neutral and unlinked polymorphic microsatellite loci were developed for this species and two additional loci were found to cross-amplify from a related species. Nine loci conformed to Hardy–Weinberg expectations. Seven loci cross-amplified with Chorocaris sp. 2, an alvinocaridid shrimp found at vents in the Southwestern Pacific. Microsatellite loci developed for R. hybisae are being deployed to study connectivity and genetic variability of populations along the Mid-Cayman Spreading Center.
KeywordsMid-Cayman Spreading Center Hydrothermal vent Rimicaris hybisae Chorocaris Alvinocarididae
This research was supported by NSF award OCE-1,031,050 and NASA ASTEP subcontract through WHOI (NNX09AB75G) to C.L. Van Dover. We are truly thankful to the Chief scientist, C. German, the crew of the R/V Altantis (18th voyage) and the crew of the ROV Jason. We acknowledge Mengyou Wu for helping with the DNA extraction of the R. hybisae individuals and Kevin Zelnio for helping with the DNA extraction of the Chorocaris individuals. SP, ADT, CLVD conceived the study; SP, CLVD collected samples; AJ undertook molecular benchwork; AJ, SP, ADT analyzed the data; AJ, SP, ADT and CLVD drafted the manuscript. All authors have read and approved the final manuscript.
- Connelly DP, Copley JT, Murton BJ, Stansfield K, Tyler PA, German CR, Van Dover CL, Amon D, Furlong M, Grindlay N, Hayman N, Huhnerback V, Judge M, Le Bas T, McPhail S, Meier A, Nakamura K-I, Nye V, Pebody M, Pederson RB, Plouviez S, Sands C, Searle RC, Taws S, Wilcox S (2012) Hydrothermal vent fields and chemosynthetic biota on the world’s deepest seafloor spreading centre. Nat Commun 3:620. doi: 10.1038/ncomms1636 PubMedCrossRefGoogle Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJGoogle Scholar