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Development of 24 new microsatellite markers in the Crested Serpent Eagle (Spilornis cheela hoya)

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Abstract

Twenty-four microsatellite markers were developed for the Crested Serpent Eagle (Spilornis cheela hoya) composing a new marker set. The markers were tested on 61 Crested Serpent Eagle individuals. The number alleles ranged from 2 to 8 per locus (average = 3.8), and the effective number of alleles ranged from 1.13 to 6.07 (average = 2.34). In the new marker set, there were 11 markers with high polymorphism (PIC > 0.5), and the average HE and HO over all loci was 0.50 and 0.49, respectively. The results showed that the new marker set was highly polymorphic as an individual genetic marker compared to the existing cross-species markers previously used for population genetic structure monitoring of the Taiwan Crested Serpent Eagle. In conclusion, the microsatellite marker set developed can be applied as a molecular tool to investigate the genetic structure or phylogeny of the Crested Serpent Eagle.

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Acknowledgments

We thank Taipei Zoo, Endemic Species Research Institute and National Taiwan Museum for supplying Crested Serpent Eagle samples. Our gratitude also goes to Dr. Harry Mersmann for his comments and English editing on the manuscript, and to Hung-Wen Lin and Yi-Hui Wang for technical assistance. This work was supported in part by grants from Taipei Zoo (Grant No. 98-AR-6 and 99-AR-9).

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Authors and Affiliations

  1. Department of Animal Science and Technology, National Taiwan University, No. 50, Lane155, Sec. 3, Keelung Rd., Taipei, 10673, Taiwan

    Hsin-Hsin Hsu, Shih-Torng Ding, Yi-Ying Chang, Chi-Chen Hsu & Pei-Hwa Wang

  2. Taipei Zoo, No. 30, Sec. 2, Xinguang Rd., Taipei, 11656, Taiwan

    Ming-Chieh Chao & Hsien-Shao Tsao

  3. Endemic Species Research Institute, Council of Agriculture, No. 1, Ming-shen East Rd., Chichi Township, Nantou County, 552, Taiwan

    Fang-Tse Chan

  4. School of Forestry and Resource Conservation, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan

    Hsiao-Wei Yuan

Authors
  1. Hsin-Hsin Hsu
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  2. Shih-Torng Ding
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  3. Yi-Ying Chang
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  4. Ming-Chieh Chao
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  5. Hsien-Shao Tsao
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  7. Chi-Chen Hsu
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  8. Hsiao-Wei Yuan
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  9. Pei-Hwa Wang
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Corresponding author

Correspondence to Pei-Hwa Wang.

Additional information

H.-H. Hsu and S.-T. Ding contributed equally to this paper.

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Hsu, HH., Ding, ST., Chang, YY. et al. Development of 24 new microsatellite markers in the Crested Serpent Eagle (Spilornis cheela hoya). Conservation Genet Resour 5, 417–420 (2013). https://doi.org/10.1007/s12686-012-9817-0

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  • DOI: https://doi.org/10.1007/s12686-012-9817-0

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