Development and characterization of polymorphic microsatellite marker for East Asian species of the genus Plestiodon
We isolated and characterized nine microsatellite markers for Plestiodon kishinouyei and Plestiodon marginatus, which are of conservation concern, with sever population decline. The number of alleles and expected heterozygosity at each locus in P. kishinouyei were 1–15 and 0.000–0.910, respectively, and those in P. marginatus were 1–9 and 0.000–0.842, respectively. Cross-amplification in five other congeners in East Asia confirmed that these markers are applicable to almost all of those species and widley useful for genetic analyses of the East Asian Plestiodon.
KeywordsPlestiodon East Asia Microsatellite Multiplex PCR Fluorescently labelled universal primer
We would like to thank S. Yamamoto, M. Inoue-Murayama, E. Inoue, B.B. Kayang, T. Kishida, E. Kawaguchi, K. Kato, T. Okamoto, and A. Hayano for their technical support and their kind advice; T. Sasai, Y. Kadota, and T. Nakano for collecting samples. This research was financially supported in part by the Global COE Program A06 to Kyoto University and by the Grant from the Japan Society for the Promotion of Science (JSPS KAKENHI Grant Number 22570094 to M. Toda). Collecting samples of P. kishinouyei was carried out under the financial support and permission from the Okinawa Prefectural Board of Education.
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