Advertisement

Conservation Genetics Resources

, Volume 5, Issue 2, pp 355–357 | Cite as

Development and characterization of polymorphic microsatellite marker for East Asian species of the genus Plestiodon

  • Kazuki Kurita
  • Tsutomu Hikida
  • Mamoru Toda
Technical Note

Abstract

We isolated and characterized nine microsatellite markers for Plestiodon kishinouyei and Plestiodon marginatus, which are of conservation concern, with sever population decline. The number of alleles and expected heterozygosity at each locus in P. kishinouyei were 1–15 and 0.000–0.910, respectively, and those in P. marginatus were 1–9 and 0.000–0.842, respectively. Cross-amplification in five other congeners in East Asia confirmed that these markers are applicable to almost all of those species and widley useful for genetic analyses of the East Asian Plestiodon.

Keywords

Plestiodon East Asia Microsatellite Multiplex PCR Fluorescently labelled universal primer 

Notes

Acknowledgments

We would like to thank S. Yamamoto, M. Inoue-Murayama, E. Inoue, B.B. Kayang, T. Kishida, E. Kawaguchi, K. Kato, T. Okamoto, and A. Hayano for their technical support and their kind advice; T. Sasai, Y. Kadota, and T. Nakano for collecting samples. This research was financially supported in part by the Global COE Program A06 to Kyoto University and by the Grant from the Japan Society for the Promotion of Science (JSPS KAKENHI Grant Number 22570094 to M. Toda). Collecting samples of P. kishinouyei was carried out under the financial support and permission from the Okinawa Prefectural Board of Education.

References

  1. Blacket MJ, Robin C, Good RT, Lee SF, Miller AD (2012) Universal primers for fluorescent labeling of PCR fragments—an efficient and cost-effective approach to genotyping by fluorescence. Mol Ecol Notes 12:456–463Google Scholar
  2. Brandley MC, Ota H, Hikida T, Nieto Montes de Oca A, Feria-Ortiz M, Guo X, Wang Y (2012) The phylogenetic systematics of blue-tailed skinks (Plestiodon) and the family Scincidae. Zool J Linn Soc 165:163–189CrossRefGoogle Scholar
  3. Coughlan JP, Kitson L, Dillane E, Davenport J, Cross TF (2004) Characterization of six microsatellite loci in the Bermuda skink (Eumeces longirostris). Mol Ecol Notes 4:678–679CrossRefGoogle Scholar
  4. Feria-Ortiz M, García-Vázquez UO (2012) A new species of Plestiodon (Squamata: Scincidae) from Sierra Madre del Sur of Guerrero, México. Zootaxa 3339:57–68Google Scholar
  5. Frankham R, Ballou JD, Briscoe DA (2002) Intoroduction to conservation genetics. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  6. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222PubMedCrossRefGoogle Scholar
  7. Howes BJ, Lee-Yaw A, Robertson AV, Bogart JP, Lougheed SC (2004) Microsatellite loci from the five-lined skink (Eumeces fasciatus). Mol Ecol Notes 4:237–238CrossRefGoogle Scholar
  8. Howes BJ, Lindsay B, Lougheed SC (2006) Range-wide phylogeography of temperate lizard, the five-lined skink (Eumeces fasciatus). Mol Phylogenet Evol 40:183–194PubMedCrossRefGoogle Scholar
  9. IUCN (2012) IUCN red list of threatened species. Version 2012.2. www.iucnredlist.org. Accessed 18 Oct 2012
  10. Kurita K, Kadota Y (2010) Current status of Plestiodon marginatus on Okinawajima Island. Jpn Akamata 21:39–43 (in Japanese)Google Scholar
  11. Okamoto T, Hikida T (2012) A new cryptic species allied to Plestiodon japonicus (Peters, 1864) (Squamata: Scincidae) from eastern Japan, and diagnoses of the new species and two parapatric congeners based on morphology and DNA barcode. Zootaxa 3436:1–23Google Scholar
  12. Ota H (2000) Eumeces kishinouyei. In: Environment Agency of Japan (ed) Threatened wildlife of Japan—red data book, 2nd edn. reptilia/amphibia. Japan Wildlife Research Center, Tokyo, p 58 (in Japanese)Google Scholar
  13. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295CrossRefGoogle Scholar
  14. Reid DT, Ashton KG, Zamudio KR (2004) Characterization of microsatellite markers in the threatened sand skink (Neoseps reynoldsi). Mol Ecol Notes 4:691–693CrossRefGoogle Scholar
  15. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225CrossRefGoogle Scholar
  16. Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106PubMedCrossRefGoogle Scholar
  17. Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
  18. Schuelke M (2000) An economic method for fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar
  19. Toyama M (2005) Eumeces kishinouyei. In: Nature Conservation Division, Department of Culture and Environment Affairs, Okinawa Prefectural Government (ed) Threatened wildlife in Okinawa, 2nd edn. (animals)—red data Okinawa. Nature Conservation Division, Department of Culture and Environment Affairs, Okinawa Prefectural Government, Naha, pp 121–122 (in Japanese)Google Scholar
  20. Wildlife Devision, Nature Conservation Bureau, Japan Ministry of Environment (WDNCBJ) (2010) Annotated appendix of the revised red list of Japan (reptilia and amphibia). Wildlife Division, Nature Conservation Bureau, Japan Ministry of Environment. http://www.biodic.go.jp/rdb/explanatory_pdf/21amphibian_reptile.pdf. Accessed 18 Oct 2012 (in Japanese)

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Department of Zoology, Graduate School of ScienceKyoto UniversityKyotoJapan
  2. 2.Tropical Biosphere Research CenterUniversity of the RyukyusNishihara, OkinawaJapan

Personalised recommendations