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Isolation and characterization of polymorphic microsatellite loci in the mahseer Neolissochilus soroides (Teleostei: Cyprinidae) from Khao Soi Dao population in Thailand

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Abstract

Habitat fragmentation and anthropogenic activities have caused changes to many fishes, such as Neolissochilus soroides in protected areas despite of its abundance. Therefore, it would be interesting to observe the changes in behavior and genetics of this species at fine scale. Quantification of genetic diversity in N. soroides using primers from related species was not successful. Therefore, new microsatellite loci were developed. The number of alleles per locus ranged from 3 to 17 with an average of 8.21 alleles per locus. Significant linkage disequilibrium was not observed. Deviation from Hardy–Weinberg equilibrium was observed at several loci, which coincided with the detection of recent bottleneck of this population.

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Acknowledgments

We truly appreciated W.Y. Brockelman and V. Akkarapatumwong for their generosity and permission to work in their laboratories. We would like to thank the Khao Soi Dao Head Officer, I. Konlasartsanee and Mr. Von for field assistance, T. Bhummakasikara, P. Benjaponwattana, and N. Nuiplot at Institute of Molecular Biosciences for lab assistance. Permission to collect specimens was granted by the Department of National Parks, Wildlife and Plant Conservation. This work was supported by the TRF/BIOTEC Special Program for Biodiversity Research and Training grant BRT R351160.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Mahidol University, Rama VI Road, Rajathewi District, Bangkok, 10400, Thailand

    J. Khudamrongsawat

  2. Conservation Genetics and Ecology Research Group, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakorn Pathom Province, 73170, Thailand

    J. Khudamrongsawat & C. Kongrit

  3. Program in Conservation Biology, Division of Biological and Natural Resource Sciences, Mahidol University at Kanchanaburi Campus, Sai Yok, Kanchanaburi Province, 71150, Thailand

    C. Siripunkaw

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  1. J. Khudamrongsawat
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  2. C. Kongrit
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  3. C. Siripunkaw
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Correspondence to J. Khudamrongsawat.

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Khudamrongsawat, J., Kongrit, C. & Siripunkaw, C. Isolation and characterization of polymorphic microsatellite loci in the mahseer Neolissochilus soroides (Teleostei: Cyprinidae) from Khao Soi Dao population in Thailand. Conservation Genet Resour 5, 351–353 (2013). https://doi.org/10.1007/s12686-012-9801-8

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  • DOI: https://doi.org/10.1007/s12686-012-9801-8

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