Skip to main content
SpringerLink
Log in

Development of ten polymorphic microsatellite loci for three closely related chameleons in Madagascar: Furcifer lateralis, Furcifer major, and Furcifer viridis

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

The three chameleon species Furcifer lateralis, Furcifer viridis, and Furcifer major are endemic to Madagascar, and are of conservation importance because they are exported in high numbers in the pet trade. These species represent a recently evolved species complex, with relatively low levels of genetic differentiation for nuclear genes. We here characterize 10 polymorphic microsatellite loci that are variable across the three species. These loci are the first microsatellites to be developed for any Malagasy chameleon. Allelic diversity greatly differs among loci, ranging from 4 to 40. This is also true of observed heterozygosity, with values ranging from 0 to 0.96. These microsatellite loci are expected to be useful for detecting potential hybridization at the contact zones between these species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Boutin-Ganache I, Raposo M, Raymond M, Deschepper CF (2001) M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. Biotechniques 31:24–28

    PubMed  CAS  Google Scholar 

  • Castelo AT, Martins W, Gao GR (2002) TROLL—tandem repeat occurrence locator. Bioinformatics 18:635–636

    Article  Google Scholar 

  • Florio AM, Ingram CM, Rakotondravony HA, Louis EE Jr, Raxworthy CJ (2012) Detecting cryptic speciation in the widespread and morphologically conservative carpet chameleon (Furcifer lateralis) of Madagascar. J Evolut Biol 25:1399–1414

    Article  CAS  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Met Enzymol 395:202–222

    Article  CAS  Google Scholar 

  • Hartl DL (1999) A primer of population genetics (3rd edn). Sinauer Associates, Inc., Massachusetts

    Google Scholar 

  • Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in excel. Population genetic software for teaching and research—an update. Bioinformatics 28:2537–2539

    Article  PubMed  CAS  Google Scholar 

  • Rousset F (2008) Genepop‘007: a complete reimplementation of the genepop software for windows and linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We thank Susan Perkins and the lab technicians at the AMNH Sackler Institute for Comparative Genomics laboratory for their help with developing the microsatellite library. This project was funded by the U.S. National Science Foundation (DEB 0641023, DEB 1110593).

Author information

Authors and Affiliations

  1. Richard Gilder Graduate School, American Museum of Natural History, Central Park West at 79th Street, New York, NY, 10024, USA

    Antonia M. Florio, Bryan G. Falk & Christopher J. Raxworthy

Authors
  1. Antonia M. Florio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bryan G. Falk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christopher J. Raxworthy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Antonia M. Florio.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Florio, A.M., Falk, B.G. & Raxworthy, C.J. Development of ten polymorphic microsatellite loci for three closely related chameleons in Madagascar: Furcifer lateralis, Furcifer major, and Furcifer viridis . Conservation Genet Resour 5, 343–345 (2013). https://doi.org/10.1007/s12686-012-9799-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-012-9799-y

Keywords

Search

Navigation