Development and characterization of thirteen microsatellite loci in Clark’s nutcracker (Nucifraga columbiana)

Abstract

Clark’s nutcrackers are important seed dispersers for two widely-distributed western North American conifers, whitebark pine and limber pine, which are declining due to outbreaks of mountain pine beetle and white pine blister rust. Because nutcracker seed dispersal services are key to maintaining viable populations of these imperiled pines, knowledge of movement patterns of Clark’s nutcrackers helps managers understand local extinction risks for these trees. To investigate population structure within Clark’s nutcracker, we developed primers for and characterized 13 polymorphic microsatellite loci. In a screen of 22 individuals from one population, levels of variability ranged from 6 to 15 alleles. No loci were found to be linked, although 4 loci revealed significant departures from Hardy–Weinberg equilibrium and evidence of null alleles. These microsatellite loci will enable population genetic analyses of Clark’s nutcrackers, which could provide insights into the spatial relationships between nutcrackers and the trees they help disperse.

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Acknowledgments

This work was funded by a grant to DFT and MBW from the College of Arts and Sciences Research Innovation Seed Program (CRISP), University of Colorado Denver. We thank Alan Kamil (University of Nebraska), Russell Balda, and Russell Benford (Northern Arizona University) for the Clark’s Nutcracker specimen. The use of any trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

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Correspondence to Sara J. Oyler-McCance.

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Oyler-McCance, S.J., Fike, J.A., Castoe, T.A. et al. Development and characterization of thirteen microsatellite loci in Clark’s nutcracker (Nucifraga columbiana). Conservation Genet Resour 5, 303–305 (2013). https://doi.org/10.1007/s12686-012-9789-0

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Keywords

  • Clark’s nutcracker
  • Microsatellites
  • Nucifraga columbiana
  • Pinus albicaulis
  • Population genetics
  • Whitebark pine