Abstract
72 microsatellite loci were isolated from a (GATA)8 enriched genomic library of Schizothorax wangchiachii (Fang), an endemic fish species in the upper reaches of the Yangtze River. A population of 32 wild captured individuals of this species was tested by these 72 primer pairs developed, 11 pairs of which exhibited polymorphic. The number of observed alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.344 to 0.875 and from 0.289 to 0.875, respectively. No significant linkage disequilibrium was detected among the loci. One loci showed significant deviation from Hardy–Weinberg equilibrium and none was detected in linkage equilibrium. These microsatellite loci described for S. wangchiachii will be useful for future study on population structure, breeding, and conservation studies.
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References
Cao WX, Chen YY, Wu YF, Zhu SQ (1981) Origin and evolution of schizothoracinae fishes in relation to the upheaval of the Xizang Plateau. In: The Team of the Comprehensive Scientific Expedition to the Qinghai-Xizang Plateau, Chinese Academy of Sciences (ed) Collection in studies on the period, amplitude and type of the uplift of the Qinghai-Xizang plateau. Science Press, Beijing, pp 118–130
Chen DQ, Chang JB, Gu HB (2005) Impacts of Jinsha River first stage project on ecology and environment of nature reserve and its countermeasures. J Yangtze River Sci Res Inst 22(2):21–24
Ding R (ed) (1994) The fishes of Sichuan, China. Sichuan Publishing House of Science and Technology, Chengdu, Sichuan, China
Liu Y, Leng Y, Xu W, Bao J, Yang G, Wang D (2007) Preliminary study on artificial propagation of Schizothorax wangchiachii. Reserv Fish 27(5):31–32
Meng L, Zhang J (2007) RAPD genetic relationship analysis of five (subspecies) schizothoracine fish in the Yalong River. China Fish 6:73–76
Oosterhout CV, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotypingerrors in microsatellite data. Mol Ecol Notes 4:535–538
Ren L, Zhu BQ, Zhang YB, Wang HY, Li CY, Su YH, Ba CF (2004) The research of applying primer premier 5.0 to design PCR primer. J Jinzhou Med Coll 25:43–46
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106
Sambrook J, Russell DW (2001) Molecular cloning, 3rd edn. Cold Spring Harbor Laboratory Press, NewYork
Xu W, Leng Y, Liu Y, Bao J, Yang Z, Meng Z, Zhang G (2003) Sdudy on artificial domestication experiment of Schizothorax wangchiachii. Reserv Fish 23(3):16–17
Yeh FC, Yang RC, Boyle T (1999) POPGENE Version l.31, microsoft window-based freeware for population genetic analysis. University of Alberta and Centre for International Forestry Research, Edmonton
Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16
Zhu B, Liao X, Shao Z, Rosenthal H, Chang J (2005) Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis. Mol Ecol Notes 5:888–892
Acknowledgments
This project was parts of a long term investigation on the status of endemic fishes in the upper reaches of Yangtze River initiated from 2011 to 2018. The work was supported by research grants from National Ministry of scientific and technology (2008DFA31550), NSFC (51109145) and China Three Gorges Corporation Scientific Project (0799527).
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Li, W., Pan, L., Que, Y. et al. Isolation and characterization of 11 microsatellite loci in Schizothorax wangchiachii (Fang). Conservation Genet Resour 5, 271–273 (2013). https://doi.org/10.1007/s12686-012-9785-4
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DOI: https://doi.org/10.1007/s12686-012-9785-4