Development of microsatellite loci for the western slimy salamander (Plethodon albagula) using 454 sequencing
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Terrestrial salamanders of the genus Plethodon are closely tied to abiotic environmental habitat features and are particularly sensitive to land use and habitat change. To better understand the effects of land use and habitat fragmentation on population genetic characteristics, we screened 137 primers developed from 454 sequence libraries, and developed 27 microsatellite primers for the western slimy salamander, Plethodon albagula. Twenty-two of these primers cross-amplified in P. shermani. These new loci will be invaluable tools to study habitat variables affecting gene flow, population genetic structure, and genetic diversity across heterogeneous landscapes.
KeywordsPlethodon shermani Population genetics Landscape genetics
We thank C. Cramer (ASU) for sharing 454 sequences for P. albagula, and funding from the University of Missouri Research Board (CB000402), the DoD Strategic Environmental Research and Development Program (RC2155), the Arkansas Biosciences Institute (research component of Arkansas Tobacco Settlement Proceeds Act of 2000), National Science Foundation (DEB-0949590 to KHK), and S.E. Trauth for Arkansas field site access.
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