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A targeted gene approach to SNP discovery in the White Rhino (Ceratotherium simum)

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Abstract

We report the characterization of 10 single nucleotide polymorphism (SNP) markers for the White Rhino (Ceratotherium simum), based on a targeted gene approach. The polymorphisms of these SNP loci were assessed using a captive population comprising 30 individuals. The minor allele frequency ranged from 0.256 to 0.413 and the observed and expected heterozygosity ranged from 0.05 to 0.37 and from 0.05 to 0.49, respectively. An understanding of genetic population structure is required to effectively formulate strategies for conservation and/or management. These SNP markers could be employed to provide estimates of parameters such as population structure, relatedness and current and historical gene flow.

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Acknowledgments

We thank the Advanced Biomolecular Research Cluster at the University of the Free State for research funding.

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Authors and Affiliations

  1. Department of Genetics, University of the Free State, P.O. Box 339, Bloemfontein, 9300, South Africa

    Christiaan Labuschagne, Antoinette Kotzé, J. Paul Grobler & Desiré L. Dalton

  2. National Zoological Gardens of South Africa, P.O. Box 754, Pretoria, 0001, South Africa

    Antoinette Kotzé & Desiré L. Dalton

  3. Inqaba Biotechnical Industries (Pty) Ltd, P.O. Box 14356, Hatfield, 0028, South Africa

    Christiaan Labuschagne

Authors
  1. Christiaan Labuschagne
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  2. Antoinette Kotzé
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  3. J. Paul Grobler
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  4. Desiré L. Dalton
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Correspondence to Desiré L. Dalton.

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Labuschagne, C., Kotzé, A., Paul Grobler, J. et al. A targeted gene approach to SNP discovery in the White Rhino (Ceratotherium simum). Conservation Genet Resour 5, 265–266 (2013). https://doi.org/10.1007/s12686-012-9783-6

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  • DOI: https://doi.org/10.1007/s12686-012-9783-6

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