Abstract
The beach wolf spider, Arctosa littoralis, inhabits areas near water. A technique based on 454 sequencing was used for obtain microsatellite loci for the wolf spiders. Fourteen polymorphic microsatellites were designed and tested as specific markers for the species. All markers were genotyped on 36 individuals from Las Playitas on the Baja California Peninsula. We observed low to medium genetic variation across most loci (with a mean of seven alleles per locus). Expected heterozygosity across all markers was considered intermediate (mean He = 0.57, range 0.05–0.91). These loci are the first to be characterized explicit for A. littoralis and should be useful for investigation of population structure and gene flow in this species.
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Acknowledgments
We thank Emiliano Mendez, Veronica Castañeda and Carlos Palacios for field assistance. Ira Fogel of CIBNOR provided valuable editing services. Funding was provided by the Consejo Nacional de Ciencia y Tecnología (CONACYT project CB-2008-01-106925). The Mexican government kindly issued permits (SGPA/DGVS/10182/11) to conduct this research. S.E.B. is a recipient of a CONACYT student fellowship (224242). FJGdL received a grand during Sabbatical stay from CONICYT, Chile (MEC-122 2010/8010014) and logistical support from CEQUA Foundation of Chile.
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Esquivel-Bobadilla, S., Adrian Lozano-Garza, O., García-De-León, F.J. et al. Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing. Conservation Genet Resour 5, 261–263 (2013). https://doi.org/10.1007/s12686-012-9782-7
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DOI: https://doi.org/10.1007/s12686-012-9782-7