Conservation Genetics Resources

, Volume 5, Issue 1, pp 243–249 | Cite as

Development and cross-amplification of thirty microsatellite loci in five diploid and polyploid Central Asian species of Palearctic green toads (Bufo viridis subgroup)

  • Caroline Betto-Colliard
  • Roberto Sermier
  • Nicolas Perrin
  • Matthias StöckEmail author
Technical Note


We report 30 polymorphic microsatellite markers for five species of Palearctic green toads (Bufo viridis subgroup): 23 in the diploid B. latastii, 19 in diploid B. turanensis, 20 in diploid B. shaartusiensis, 27 in tetraploid B. pewzowi, and 30 in triploid B. baturae. Genetic diversity at these loci, measured for 10–18 individuals per species, ranged from 2 to 19 alleles. These microsatellite loci will be useful for conservation plans (genetic diversity, population structure, evolutionary units), inheritance patterns, and evolution of green toads.


Bufo viridis subgroup Microsatellite markers Polyploidy Hybrid speciation 



We thank Simon Cawsey, Valery Eremchenko, Stefan Michel, and Bakhtiyor Rasulov for help in the field in Kyrgyzstan and Tajikistan, and Françoise Dolivo for help in the laboratory. This work was funded in part by a grant from the Fondation Agassiz of the University of Lausanne (1 July 2010) and a Heisenberg-Fellowship (Sto 493/2-1) and grant STO 493/3-1 of the Deutsche Forschungsgemeinschaft (DFG), all to MS.


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Caroline Betto-Colliard
    • 1
  • Roberto Sermier
    • 1
  • Nicolas Perrin
    • 1
  • Matthias Stöck
    • 2
    Email author
  1. 1.Department of Ecology and EvolutionUniversity of LausanneLausanneSwitzerland
  2. 2.Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB)BerlinGermany

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