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Isolation of nuclear microsatellite loci in the African tree Scorodophloeus zenkeri (Fabaceae)

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Abstract

Scorodophloeus zenkeri is a tropical tree from mature rainforests of Central Africa, locally threatened by overexploitation. Ten nuclear microsatellite markers, amplifiable in two multiplexed reactions, were developed and characterized in one population. The levels of polymorphism observed (3–15 alleles per locus, expected heterozygosity ranging from 0.198 to 0.905) point at the promising application of the markers for conservation purposes regarding the location of past forest refugia, assessment of mating system and evaluation of genetic diversity in isolated and threatened populations.

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References

  • Abanda GN (2011) La Surexploitation du Scorodophloeus zenkeri Harms (Arbre à ail): Atténuer l’impact de la gestion non durable de l’arbre à ail dans le massif forestier de Ngovayang du Sud Cameroun. Editions Universitaires Europeennes

  • Chybicki IJ, Burczyk J (2009) Simultaneous estimation of null alleles and inbreeding coefficients. The J Hered 100: 106–113 (www.genetyka.ukw.edu.pl/index_pliki/software.htm)

    Google Scholar 

  • Culley T, Weller MSG, Sakai AK, Putnam KA (2008) Characterization of microsatellite loci in the Hawaiian endemic shrub Schiedea adamantis (Caryophyllaceae) and amplification in related species and genera. Mol Ecol Resour 8:1081–1084

    Article  PubMed  CAS  Google Scholar 

  • Dauby G (2012) Structuration spatiale de la diversite intra- et interspecifique en Afrique centrale–Le cas des forêts gabonaises. PhD thesis. Université Libre de Bruxelles, Belgium

  • Duminil J, Koffi GK, Debout G, Sebastiani F, Vendramin GG, Heuertz M, Gonzalez-Martinez SC, Hardy OJ (2011) Isolation of SSR markers for two African tree tropical species Erythrophleum suaveolens and E. ivorense (Caesalpinioideae). Am J Bot 98:E106–E108

    Article  PubMed  CAS  Google Scholar 

  • Hardy OJ, Vekemans X (2002) SPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol Ecol Notes 2:618–620 (www.ebe.ulb.ac.be/ebe/Software.html)

  • Malausa T, Gilles A, Meglécz E, Blanquart H, Duthoy S, Costedoat C, Dubut V et al (2011) High-throughput microsatellite isolation through 454 GS-FLX titanium pyrosequencing of enriched DNA libraries. Mol Ecol Resour 11:638–644

    Article  PubMed  CAS  Google Scholar 

  • Micheneau C, Dauby G, Bourland N, Doucet J-L, Hardy OJ (2011) Development and characterization of microsatellite loci in Pericopsis elata (Fabaceae) using a cost-efficient approach. Am J Bot 98:e268–e270

    Article  PubMed  CAS  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249 (www.genepop.curtin.edu.au/)

  • Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was financed by the Fund for Scientific Research of Belgium (F.R.S.-FNRS, grant MIS F.4.519.10.F). We acknowledge Thibaut Malausa for allowing us to benefit from the project ‘EcoMicro’ (AIP BioRessources grant, INRA) and the R&D budget of Genoscreen (Lille, France).

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Correspondence to Rosalía Piñeiro.

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Piñeiro, R., Micheneau, C., Dauby, G. et al. Isolation of nuclear microsatellite loci in the African tree Scorodophloeus zenkeri (Fabaceae). Conservation Genet Resour 5, 219–221 (2013). https://doi.org/10.1007/s12686-012-9773-8

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  • DOI: https://doi.org/10.1007/s12686-012-9773-8

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