Abstract
This study describes the comparison of three DNA extraction protocols for successful extraction of PCR amplifiable quality DNA from bones, antlers and feces samples of Sambar deer (Rusa unicolor). Three different DNA extraction protocols were compared in this study including Phenol–Chloroform (PC), column based Qiagen kit, and Guanidine hydrochloride (Gu-HCl) based in-house method. The effectiveness of the protocols was compared for higher success rate of PCR amplification from the extracted DNA. This study highlights that silica based indigenous DNA extraction protocol using Gu-HCl chaotropic salts yields better quality DNA with higher PCR amplification success rate.
This is a preview of subscription content, log in via an institution to check access.
References
Gupta SK, Verma SK, Singh L (2005) Molecular insight into a wildlife crime: the case of a peafowl slaughter. Forensic Sci Int 154:214–217
Gupta SK, Thangaraj K, Singh L (2011) Identification of the source of ivory idol by DNA analysis. J Forensic Sci 56:1343–1345
Gupta SK, Thangaraj K, Singh L (2012) Molecular insight into suspected food crime cases: a study to determine the legitimacy of meat by DNA typing. Biol Biomed Rep 2:171–174
Höss M, Pääbo S (1993) DNA extraction from Pleistocene bones by a silica-based purification method. Nucleic Acids Res 21:3913–3914
Rohland N, Hofreiter M (2007a) Ancient DNA extraction from bones and teeth. Nat Protoc 2:1756–1762
Rohland N, Hofreiter M (2007b) Comparison and optimization of ancient DNA extraction. Biotechniques 42:343–352
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Verma SK, Singh L (2003) Novel universal primers establish identity of enormous number of animal species for forensic application. Mol Ecol Notes 3:28–31
Wehausen JD, Ramey RR II, Epps CW (2004) Experiments in DNA extraction and PCR amplification from bighorn sheep faeces: the importance of DNA extraction method. J Hered 95:503–509
Acknowledgments
This work was funded by the Ministry of Environment and Forest, Govt. of India through the Grant-in-Aid to WII. We thank to the Director, Dean, and Nodal Officer (Forensic Cell) of WII for their support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gupta, S.K., Kumar, A. & Hussain, S.A. Extraction of PCR-amplifiable DNA from a variety of biological samples with uniform success rate. Conservation Genet Resour 5, 215–217 (2013). https://doi.org/10.1007/s12686-012-9772-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-012-9772-9