Abstract
We report on the development of 36 novel polymorphic microsatellite markers from the bar-shouldered skink, Ctenotus inornatus. Members in this genus account for up to 20 % of the diverse Australian lizard species. Microsatellite markers were developed to investigate island endemism amongst Kimberley islands taxa. We describe the diversity from 110 adult Ctenotus individuals. Overall, the markers produced between 9 to 40 alleles per locus and observed and expected heterozygosities of 74.6 and 95.7 %, respectively. Due to island endemism, habitat modification by introduced grazing mammals, some urban encroachment and changes in fire regime there has been fragmentation of its range. These microsatellite markers are an important tool for evaluating population genetic information and for prioritising populations for conservation management.
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Acknowledgments
The project was funded by the Department of Environment and Conservation, Murdoch University and the WA Museum. The authors wish to thank Paul Doughty, Claire Stevenson and Linette Umbrello for their assistance.
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Harradine, E., How, R.A., Schmitt, L.H. et al. Isolation and characterisation of 36 polymorphic microsatellite markers using 454 sequencing in the bar-shouldered skink, Ctenotus inornatus . Conservation Genet Resour 5, 207–210 (2013). https://doi.org/10.1007/s12686-012-9769-4
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DOI: https://doi.org/10.1007/s12686-012-9769-4