Abstract
Several Australian arid zone Acacia species are under threat because of decades of fruiting and recruitment failure that may reflect the loss of genetic diversity within small and isolated populations. We developed primers for eight microsatellite loci for Acacia carneorum and Acacia loderi. We detected high levels of clonality in each of two stands of A. carneorum (1 and 2 genets). In contrast, one stand of A. loderi was wholly clonal (1 genet), while in a second there were 30 unique genotypes. These loci allow assessment of the genetic diversity and connectedness of populations, the relative contribution of asexual reproduction to genotypic diversity and population structure, and use of paternity analysis to identify sires of seed within populations known to have set seed in past decades. This type of information may provide a basis for a recovery plan based on ‘genetic rescue’.
References
Arnaud-Haond S, Belkhir K (2007) GENCLONE: a computer program to analyse genotypic data, test for clonality and describe spatial clonal organization. Mol Ecol Notes 7:15–17
Arnaud-Haond S, Duarte CM, Alberto F, Serrao EA (2007) Standardizing methods to address clonality in population studies. Mol Ecol 16:5115–5139
Auld TD (1993) The impact of grazing on regeneration of the shrub Acacia carnei in arid Australia. Biol Conserv 65:165–176
Auld TD (1995) Soil seedbank patterns of four trees and shrubs from arid Australia. J Arid Environ 29:33–45
Auld TD, Denham AJ (2001) Flora conservation issues at Kinchega National Park, western NSW. Cunninghamia 7:27–41
Chapman AR, Maslin BR (2001) Acacia loderi. Flora of Australia, vol 11B, Mimosaceae, Acacia part 2. Australian Biological Resources Study/CSIRO Publishing, p. 107
Crisp MD, Lange RT (1976) Age structure, distribution and survival under grazing of the arid-zone shrub Acacia burkittii. Oikos 27:86–92
Cunningham SA (2000a) Depressed pollination in habitat fragments causes low fruit set. Proc R Soc B Biol Sci 267:1149–1152
Cunningham SA (2000b) Effects of habitat fragmentation on the reproductive ecology of four plant species in mallee woodland. Conserv Biol 14:758–768
Denham AJ, Auld TD (2004) Survival and recruitment of seedlings and suckers of trees and shrubs of the Australian arid zone following habitat management and the outbreak of Rabbit Calicivirus Disease (RCD). Aust Ecol 29:585–599
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
Hayden MJ, Nguyen TM, Waterman A, Chalmers KJ (2008) Multiplex-ready PCR: a new method for multiplexed SSR and SNP genotyping. BMC Genomics 9:80
Maslin BR (2001) Acacia carneorum. Flora of Australia, volume 11A, Mimosaceae, Acacia part 1. Australian Biological Resources Study/CSIRO Publishing, p. 366
Morton SR, Stafford Smith DM, Friedel MH, Griffin GF, Pickup M (1995) The stewardship of arid Australia: ecology and landscape management. J Environ Manage 43:195–217
New South Wales Threatened Species Conservation Act 1995. No 101. Accessed 6 Aug 2012. http://www.legislation.nsw.gov.au
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295
Pickup M, Young AG (2008) Population size, self-incompatibility and genetic rescue in diploid and tetraploid races of Rutidosis leptorrhynchoides (Asteraceae). Heredity 100:268–274. doi:10.1038/sj.hdy.6801070
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106
Woinarski JCZ, Fisher A (2003) Conservation and the maintenance of biodiversity in the rangelands. Rangel J 25:157–171
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Roberts, D.G., Forrest, C.N., Denham, A.J. et al. Microsatellite markers for vulnerable Australian aridzone Acacias. Conservation Genet Resour 5, 199–201 (2013). https://doi.org/10.1007/s12686-012-9767-6
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DOI: https://doi.org/10.1007/s12686-012-9767-6