Abstract
We identified 28 microsatellite loci from Popenaias popeii, a freshwater mussel that has experienced population declines throughout its range. Twenty loci were polymorphic, with 4–10 alleles, observed heterozygosity values of 0.375–1.00, and 16 % of alleles shared by more than one population. These loci should be useful for describing population genetic diversity, which will facilitate ongoing conservation efforts for P. popeii.
References
de Arruda MP, Gonçalves EC, Schneider MPC, da Costa Silva AL, Morielle-Versute E (2010) An alternative genotyping method using dye-labeled universal primer to reduce unspecific amplifications. Mol Biol Rep 37:2031–2036
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Res 8:92–94
Guichoux E, Lagache L, Wagner S, Chaumeil P, Léger P, Lepais O, Lepoittevin C, Malausa T, Revardel E, Salin F, Petit RJ (2011) Current trends in microsatellite genotyping. Mol Ecol Res 11:591–611
Howells RG, Neck RW, Murray HD (1996) Freshwater mussels of Texas. Texas Park and Wildlife Department, Austin
Karatayev AY, Miller TD, Burlakova LE (2012) Long-term changes in unionid assemblages in the Rio Grande, one of the world’s top 10 rivers at risk. Aquat Conserv: Marine Freshw Ecosyst 22:206–219
Lang BK (2001) Status of the Texas hornshell and native freshwater mussels (Unionoidea) in the Rio Grande and Pecos River of New Mexico and Texas. Completion Report, New Mexico Department of Game and Fish, Conservation Services Division, Santa Fe, New Mexico
Lessios HA (1992) Testing electrophoretic data for agreement with Hardy-Weinberg expectations. Mar Biol 112:517–523
Levine TD, Lang BK, Berg DJ (2012) Physiological and ecological hosts of Popenaias popeii (Bivalvia: Unionidae): laboratory studies identify more hosts than field studies. Freshw Biol 57:1854–1864
Lydeard C, Cowie RH, Ponder WF, Bogan AE, Bouchet P, Clark SA, Cummings KS, Frest TJ, Gargominy O, Herbert DG, Hershler R, Perez KE, Roth B, Seddon M, Strog EE, Thompson FG (2004) The global decline of nonmarine mollusks. Bioscience 54:321–330
Matschiner M, Salzburger W (2009) TANDEM: integrating automated allele binning into genetics and genomics workflows. Bioinformatics 25:1982–1983
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295
Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Res 8:103–106
Rozen S, Skaletshky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Smith DG, Lang BK, Gordon ME (2003) Gametogenetic cycle, reproductive anatomy, and larval morphology of Popenaias popeii (Unionoidea) from the Black River, New Mexico. Southwest Nat 48:333–340
Strenth NE (2004) New records of the Texas hornshell Popenaias popeii (Bivalvia: Unionidae) from Texas and northern Mexico. Texas J Sci 56:223
US Federal Register (2005) Endangered and threatened wildlife and plants; review of native species that are candidates or proposed for listing as endangered or threatened; annual notice of findings on resubmitted petitions; annual description of progress on listing actions; proposed rule. Dep Interior 70:24870–24934
van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
We thank the Plant–Microbe Genomics Facility at Ohio State University for next-generation sequencing, and staff in the Miami University Center for Bioinformatics and Functional Genomics for technical support. This study was funded by the New Mexico Department of Game and Fish.
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Inoue, K., Lang, B.K. & Berg, D.J. Development and characterization of 20 polymorphic microsatellite markers for the Texas hornshell, Popenaias popeii (Bivalvia: Unionidae), through next-generation sequencing. Conservation Genet Resour 5, 195–198 (2013). https://doi.org/10.1007/s12686-012-9766-7
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DOI: https://doi.org/10.1007/s12686-012-9766-7