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Development of microsatellite markers from four Pittosporum taxa (Pittosporaceae) endemic to the Ogasawara (Bonin) Islands with cross-amplification in Ogasawara Pittosporum and P. tobira

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Abstract

A total of 55 microsatellite loci were developed and characterized using four Pittosporum taxa endemic to the Ogasawara (Bonin) Islands, Japan: Pittosporum boninense var. boninense, P. boninense var. chichijimense, Pittosporum parvifolium var. parvifolium and P. parvifolium var. beecheyi. Transferability of these markers among the Ogasawara Pittosporum taxa as well as Pittosporum tobira was also evaluated. The number of alleles per locus ranged from 2 to 23 in P. boninense var. boninense, from 2 to 16 in P. boninense var. chichijimense, from 1 to 7 with in P. parvifolium var. parvifolium, and from 1 to 8 in P. parvifolium var. beecheyi. During cross-amplification tests, the majority of these loci were successfully amplified and showed moderate to high levels of genetic variation. The markers described here could be used to achieve a more accurate determination of genetic diversity and genetic structure of the taxa from which they were derived.

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References

  • Doyle JJ, Dickson EE (1987) Preservation of plant samples for DNA restriction endonuclease analysis. Taxon 36:715–722

    Article  Google Scholar 

  • Environment Agency of Japan (2000) Threatened wildlife of Japan. Red Data Book, 2nd edn., vol 8, Vascular Plants. Wildlife Research Center, Tokyo

  • Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3) http://www.unil.ch/izea/softwares/fstat.html. Updated from Goudet (1995)

  • Lian C, Hogetsu T (2002) Development of microsatellite markers in black locust (Robinia pseudoacasia) using a dual-suppression-PCR technique. Mol Ecol Notes 2:211–213

    CAS  Google Scholar 

  • Lian C, Wadud MA, Geng Q, Shimatani K, Hougetsu T (2006) An improved technique for isolating codominant compound microsatellite markers. J Plant Res 119:415–517

    Article  PubMed  CAS  Google Scholar 

  • Ono M, Kobayashi S, Kawakubo N (1986) Present situation of endangered plant species in the Bonin (Ogasawara) Islands. Ogasawara Res 12:1–32

    Google Scholar 

  • Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis inExcel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Toyoda T (2003) Flora of Bonin Islands (Enlarged & Revised). Aboc Press, Kamakura

    Google Scholar 

  • Untergasser A, Nijveen H, Rao X, Bisseling T, Geurts R, Leunissen JAM (2007) Primer3Plus, an enhanced web interface to Primer3. Nucleic Acid Res 35:W71–W74. doi:10.1093/nar/gkm306

    Article  PubMed  Google Scholar 

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Acknowledgments

This study was supported in part by Grants-in-Aid from the Japanese Ministry of Education, Culture, Sports, Science and Technology (No. 20241056 to H. K. and No. 2255003 to N. M.).

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Authors and Affiliations

  1. Department of Biological Science, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan

    Saeko Katoh, Noriaki Murakami & Hidetoshi Kato

  2. Botanical Gardens Koishikawa, Graduate School of Science, The University of Tokyo, 3-7-1 Hakusan, Bunkyo-ku, Tokyo, 112-0001, Japan

    Tetsuo Ohi-Toma

Authors
  1. Saeko Katoh
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  2. Tetsuo Ohi-Toma
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  3. Noriaki Murakami
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  4. Hidetoshi Kato
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Correspondence to Saeko Katoh.

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Katoh, S., Ohi-Toma, T., Murakami, N. et al. Development of microsatellite markers from four Pittosporum taxa (Pittosporaceae) endemic to the Ogasawara (Bonin) Islands with cross-amplification in Ogasawara Pittosporum and P. tobira . Conservation Genet Resour 5, 185–190 (2013). https://doi.org/10.1007/s12686-012-9764-9

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  • DOI: https://doi.org/10.1007/s12686-012-9764-9

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