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Isolation and characterization of microsatellite loci from Iguana delicatissima (Reptilia: Iguanidae), new perspectives for investigation of hybridization events with Iguana iguana

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Abstract

A (GA)n and (GT)n microsatellite-enriched library was constructed and 25 nuclear simple sequence repeat (SSR) loci were characterized in the Lesser Antillean Iguana (Iguana delicatissima). All SSR loci were found to be polymorphic after screening for diversity in different cultivars, and a cross-taxa amplification tests showed the potential transferability of most SSR markers in Iguana iguana. First to be published for I. delicatissima, this new SSR resource will be a powerful tool for intraspecific genetic studies and for investigation of hybridization events with Iguana iguana.

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Authors and Affiliations

  1. Genindexe, 17000, La Rochelle, France

    V. Valette & C. Cherbonnel

  2. Laboratoire Ecologie, Evolution, Symbiose, UMR CNRS 6556, Université de Poitiers, 40, av. du Recteur Pineau, 86022, Poitiers Cedex, France

    L. Filipová, C. Delaunay & F. Grandjean

  3. CIRAD (Centre de coopération Internationale en Recherche Agronomique pour le Développement), UMR 108 AGAP, TA A 108/03 Avenue Agropolis, 34398, Montpellier Cedex 5, France

    A. M. Risterucci

  4. Département de Systématique et d’Évolution, Amphibiens-Reptiles, Muséum national d’Histoire naturelle, 57, rue Cuvier, CP no 30, 75231, Paris Cedex 05, France

    B. Vuillaume & M. Breuil

Authors
  1. V. Valette
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  2. L. Filipová
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  3. B. Vuillaume
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  4. C. Cherbonnel
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  5. A. M. Risterucci
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  6. C. Delaunay
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  7. M. Breuil
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  8. F. Grandjean
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Correspondence to F. Grandjean.

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Valette, V., Filipová, L., Vuillaume, B. et al. Isolation and characterization of microsatellite loci from Iguana delicatissima (Reptilia: Iguanidae), new perspectives for investigation of hybridization events with Iguana iguana . Conservation Genet Resour 5, 173–175 (2013). https://doi.org/10.1007/s12686-012-9761-z

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  • DOI: https://doi.org/10.1007/s12686-012-9761-z

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