Abstract
The EPIC strategy was used in the present study in order to develop molecular markers for Epinephelus itajara. Twelve primers were designed for six genomic regions. The results of the study provide a number of appropriate new nuclear DNA markers for future genetic studies in several endangered/overexploited grouper species. The description of these markers will also expand the number of molecular tools available for the reconstruction of the life history of the different grouper species, in particular those in urgent need of effective protection measures.
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Acknowledgments
We are grateful to ICMBio and the Meros do Brasil (Brazilian Groupers) project for the samples of E. itajara. This study was supported by CNPq and FAPESPA (grant 306233/2009-6 to IS, and PRONEX 2007, respectively) and a CAPES and CNPq stipend. The authors are also grateful to CNPq for the researcher fellowship provided.
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Silva-Oliveira, G.C., Silva, A.B.C., Oliveira, Y. et al. New nuclear primers for molecular studies of Epinephelidae fishes. Conservation Genet Resour 5, 165–168 (2013). https://doi.org/10.1007/s12686-012-9759-6
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DOI: https://doi.org/10.1007/s12686-012-9759-6