Davidsonia jerseyana is an endangered rainforest tree endemic to far north-eastern New South Wales, Australia. The species occurs in small fragmented populations and has an edible plum-like fruit important to the Australian Native Food industry. Twenty one novel microsatellite markers were developed for D. jerseyana of which 13 were polymorphic for the species. Markers were characterised using 28 individuals, representing six populations from across the species geographic range. Species level analysis of the polymorphic markers revealed the mean number of alleles per locus was 3.154 (range 2–7) and mean expected and observed heterozygosities were 0.437 (range 0.035–0.725) and 0.044 (range 0–0.250) respectively. The heterozygote deficiency may indicate a predominantly selfing breeding system. All markers cross amplified in the other two Davidsonia species. These markers will be used to assess the genetic diversity, population structure and breeding systems in D. jerseyana and related taxa which will facilitate conservation management strategies.
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Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by tag DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20(6):1004–1010
Department of Environment and Conservation (NSW) (2004a) Recovery plan for Davidsonia johnsonii (Smooth Davidsonia), Hurstville
Department of Environment and Conservation (NSW) (2004b) Recovery plan for the Davidson’s Plum (Davidsonia jerseyana), Hurstville
Flanagan NS, Ebert D, Porter C, Rossetto M, Peakall R (2006) Microsatellite markers for evolutionary studies in the sexually deceptive orchid genus Chiloglottis. Mol Ecol Notes 6(1):123–126
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. In: Molecular evolution: producing the biochemical data, part B, vol 395. Methods in enzymology, pp 202–222
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6(1):288–295
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18(2):233–234
We would like to thank the Rural Industries Research and Development Corporation and NSW National Parks and Wildlife Service for funding support, The Australian Plant DNA Bank, Lismore, Australia, for access to D. johnsonii leaf samples and The Ramaciotti Centre for Gene Function Analysis (University of New south Wales, Australia) and Southern Cross Plant Genomics (Southern Cross University, Lismore, NSW, Australia) for performing sequencing and genotyping.
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Eliott, F.G., Connelly, C., Rossetto, M. et al. Novel microsatellite markers for the endangered Australian rainforest tree Davidsonia jerseyana (Cunoniaceae) and cross-species amplification in the Davidsonia genus. Conservation Genet Resour 5, 161–164 (2013). https://doi.org/10.1007/s12686-012-9758-7
- Conservation genetics
- Genetic diversity