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Characterization of novel microsatellite loci for Hetaerina americana damselflies, and cross-amplification in other species

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Abstract

Hetaerina damselflies are distributed throughout the neotropics. We developed eleven microsatellite loci for the damselfly Hetaerina americana. Microsatellites were tested for polymorphism on a panel of 24 individuals. The number of alleles ranged from 2 to 6, observed heterozygosity from 0.080 to 0.701, and the fixation index from −0.266 to 1.000. Cross-amplification was tested in 7 different species in the genus Hetaerina from the United States and Mexico. These microsatellite loci will be useful for studies of population structure and gene flow in H. americana.

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Acknowledgments

John Pollinger and the UCLA Conservation Genetic Resources center provided logistical support and helpful comments on the manuscript. John McCormack also provided helpful comments on the analysis and manuscript. CNA was supported by a CONACyT postdoctoral fellowship and a UCMEXUS-CONACyT postdoctoral fellowship during this project. Funding was provided by NSF grant DEB 1020586 to GFG.

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Authors and Affiliations

  1. Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Apdo., Postal 70-275, 04510, Mexico, D.F., Mexico

    Christopher N. Anderson

  2. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 621 Charles E. Young Drive South, Los Angeles, CA, 90095-1606, USA

    Gregory F. Grether

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  1. Christopher N. Anderson
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  2. Gregory F. Grether
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Correspondence to Christopher N. Anderson.

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Anderson, C.N., Grether, G.F. Characterization of novel microsatellite loci for Hetaerina americana damselflies, and cross-amplification in other species. Conservation Genet Resour 5, 149–151 (2013). https://doi.org/10.1007/s12686-012-9755-x

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  • DOI: https://doi.org/10.1007/s12686-012-9755-x

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