Abstract
We isolated and characterized a total of 23 microsatellite loci from the Santa Ana speckled dace, Rhinichthys osculus spp., a freshwater minnow restricted to southern California. Loci were screened in 24 individuals from five watersheds. The number of alleles per locus ranged from 7 to 25, observed heterozygosity ranged from 0.409 to 0.875, and the probability of identity values ranged from 0.005 to 0.081. These new loci will provide tools for examining taxonomic status, population structure, and management strategies.
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Acknowledgments
We wish to thank the USDA Forest Service San Bernardino, the California Department of Fish and Game and the Water Resources Institute, California State University, San Bernardino for logistical support and funding. Manuscript preparation was partially supported by the DOE under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.
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Nunziata, S.O., Lance, S.L., Jones, K.L. et al. Development and characterization of twenty-three microsatellite markers for the freshwater minnow Santa Ana speckled dace (Rhinichthys osculus spp., Cyprinidae) using paired-end Illumina shotgun sequencing. Conservation Genet Resour 5, 145–148 (2013). https://doi.org/10.1007/s12686-012-9754-y
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DOI: https://doi.org/10.1007/s12686-012-9754-y