Abstract
We describe the development based on 454 pyrosequencing technology of thirteen microsatellite markers for two closely related species of lamprey: Lampetra fluviatilis and L. planeri. The number of alleles per locus ranged from 2 to 5 in L. fluviatilis and from 2 to 6 in L. planeri. Gene diversity ranged from 0.062 to 0.718 in L. fluviatilis and from 0.322 to 0.677 in L. planeri. These markers will be helpful to study population genetic structure of both species and resolve their taxonomic status as separate species or ecotypes of a single species.
References
Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (1996–2004) GENETIX 4.05, logiciel sous windows TM pour la génétique des populations. http://www.univ-montp2.fr/~genetix/genetix.htm
Espanhol R, Almeida PR, Alves MJ (2007) Evolutionary history of lamprey paired species Lampetra fluviatilis (L.) and Lampetra planeri (Bloch) as inferred from mitochondrial DNA variation. Mol Ecol 16:1909–1924
Estoup A, Largiader CR, Perrot E, Chourrout D (1996) Rapid one-tube DNA extraction for reliable PCR detection of fish polymorphic markers and transgenes. Mol Mar Biol Biotechnol 5:295–298
Hardisty MW, Potter IC (1971) The biology of lampreys, vol 1. Academic Press, London
Lasne E, Sabatié MR, Evanno G (2010) Communal spawning of brook and river lampreys (Lampetra planeri and L. fluviatilis) is common in the Oir river (France). Ecol Freshw Fish 19:323–325
Malausa T, Gilles A, Meglécz E et al (2011) High-throughput microsatellite isolation through 454 GS-FLX titanium pyrosequencing of enriched DNA libraries. Mol Ecol Resour 11:638–644
Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin JF (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404
Renaud CB (1997) Conservation status of northern hemisphere lampreys (Petromyzontidae). J Appl Ichthyol 13:143–148
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106
Schreiber A, Engelhorn R (1998) Population genetics of a cyclostome species pair, river lamprey (Lampetra fluviatilis L.) and brook lamprey (Lampetra planeri bloch). J Zool Syst Evol Res 36:85–99
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
UICN France, MNHN, SFI, ONEMA (2010) La Liste rouge des espèces menacées en France - Chapitre Poissons d’eau douce de France métropolitaine. Paris, France. Available at www.uicn.fr/IMG/pdf/Liste_rouge_France_Poissons_d_eau_douce_de_metropole.pdf
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
We thank our colleagues from the INRA ‘U3E’ unit who helped us with samples’ collection. This study was funded by the European Regional Development Fund (Transnational program Interreg IV, Atlantic Aquatic Resource Conservation Project) and by the French Ministry of Ecology and Sustainable Development (project ‘Amphihalins, cohérence du réseau N2000 en mer’).
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Gaigher, A., Launey, S., Lasne, E. et al. Characterization of thirteen microsatellite markers in river and brook lampreys (Lampetra fluviatilis and L. planeri). Conservation Genet Resour 5, 141–143 (2013). https://doi.org/10.1007/s12686-012-9753-z
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DOI: https://doi.org/10.1007/s12686-012-9753-z