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Isolation and characterization of twenty novel microsatellite markers of Pointhead flounder (Cleisthenes herzensteini)

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Abstract

The Pointhead flounder (Cleisthenes herzensteini), a severely overexploited and endangered flatfish in the Japanese Archipelago, Bohai Sea, Yellow Sea and East China Sea, is in urgent need of conservation and management measures. In this study, a total of 20 polymorphic microsatellite markers were derived from C. herzensteini. The genotyping of 30 wild-caught individuals showed observed and expected heterozygosities ranging from 0.1724 to 1.0000 and 0.5850 to 0.9226, respectively. The average allele number was 9.5 per locus (range 5–14). These polymorphic microsatellite loci would be useful for investigating genetic diversity of C. herzensteini.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (30901098) and the National High Technology Research and Development Program of China (2012AA10A402). This study was also sponsored by K.C.Wong Magna Fund in Ningbo University.

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Authors and Affiliations

  1. Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, China

    Yajun Wang

  2. School of Marine Sciences, Ningbo University, Ningbo, China

    Yajun Wang

  3. Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, China

    Yajun Wang, Jiajun Jiang, Xubo Wang & Zhipeng Liu

Authors
  1. Yajun Wang
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  2. Jiajun Jiang
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  3. Xubo Wang
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  4. Zhipeng Liu
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Corresponding author

Correspondence to Xubo Wang.

Additional information

Yajun Wang and Jiajun Jiang contributed equally to this work.

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Wang, Y., Jiang, J., Wang, X. et al. Isolation and characterization of twenty novel microsatellite markers of Pointhead flounder (Cleisthenes herzensteini). Conservation Genet Resour 5, 137–139 (2013). https://doi.org/10.1007/s12686-012-9752-0

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  • DOI: https://doi.org/10.1007/s12686-012-9752-0

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