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New microsatellite loci isolated via next-generation sequencing for two endangered pronghorn from the Sonoran Desert

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Abstract

We isolated 16 novel microsatellite loci in two subspecies of endangered desert pronghorns (Antilocapra americana sonoriensis and Antilocapra americana peninsularis) using a shotgun pyrosequencing approach. All and 87.5 % of the loci were polymorphic within each subspecies, respectively. The mean number of alleles per locus was 4.86 (range 2–8) and 2.5 alleles per locus (range 1–4 alleles), and observed heterozygosity ranged from 0.13 to 0.78 (mean 0.48) and 0.00 to 0.61 (mean 0.31), respectively. We did not find significant linkage disequilibrium among loci pairs and only one locus deviated significantly from Hardy–Weinberg equilibrium in peninsularis.

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Acknowledgments

We deeply thank Jim Atkinson, USFWS, for initiating this study. John Hervert and Jill Bright (Arizona Game and Fish) and Ole Alcumbrac and his group of vets, for their expertise with pronghorn. To the entire team whom yearly aid to capture individuals in Ajo, Arizona. To Robert Lovich and Lisette P. Waits for their input on our genetic strategies. To Victor G. Sanchez Sotomayor, Director of Area de Proteccion de Flora y Fauna Valle de los Cirios, who contributed peninsularis samples. Mention of specific products herein does not constitute endorsement by the U.S. Geological Survey.

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Authors and Affiliations

  1. Conservation Genetics Laboratory, School of Natural Resources and Environment, BSE-317, University of Arizona, 1311 E 4th Street, Tucson, AZ, 85721, USA

    Adrian Munguia-Vega

  2. Comunidad y Biodiversidad A. C., Blvd. Agua Marina 297, entre Jaiba y Tiburon. Col. Delicias, Guaymas, 85420, Sonora, Mexico

    Adrian Munguia-Vega

  3. Departamento de Biologia Marina, Universidad Autonoma de Baja California Sur, Km 5.5 carretera al sur, La Paz, 23080, Baja California Sur, Mexico

    Anastasia Klimova

  4. U.S. Geological Survey, Arizona Cooperative Fish and Wildlife Research Unit, Conservation Genetics Laboratory, School of Natural Resources and Environment, BSE-317, University of Arizona, Tucson, AZ, 85721, USA

    Melanie Culver

Authors
  1. Adrian Munguia-Vega
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  2. Anastasia Klimova
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  3. Melanie Culver
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Correspondence to Adrian Munguia-Vega.

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Munguia-Vega, A., Klimova, A. & Culver, M. New microsatellite loci isolated via next-generation sequencing for two endangered pronghorn from the Sonoran Desert. Conservation Genet Resour 5, 125–127 (2013). https://doi.org/10.1007/s12686-012-9749-8

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  • DOI: https://doi.org/10.1007/s12686-012-9749-8

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