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Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae

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Abstract

We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the cross-amplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of long-repeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.

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Acknowledgments

We thank the University of Otago High-Throughput Sequencing Unit for use of their next-generation sequencing facility which produced the sequence reads for the development of the microsatellites. We thank Fatima Torrico, Helen Cadwaller, Ulla Kail, Sam Hill, and Damien Menard for their help in the field, and Robyn Howitt, Ana Ramón-Laca, and Diana Prada for advice in the lab. This work was supported by Massey University’s Institute of Natural Sciences, Landcare Research Ltd, and the Auckland Council.

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Authors and Affiliations

  1. Ecology and Conservation Group, Institute of Natural Sciences, Massey University, Private Bag 102904 North Shore Mail Centre, Auckland, New Zealand

    S. J. Wells

  2. Ecology and Conservation Group, Institute of Natural Sciences, Massey University, Auckland, New Zealand

    W. Ji

  3. Ocean Tracking Network Canada, Department of Biology, Life Science Centre, Dalhousie University, Halifax, Canada

    S. M. Baillie

  4. Institute of Applied Ecology, University of Canberra, Canberra, ACT, 2601, Australia

    D. Gleeson

  5. EcoGene, Landcare Research Ltd., Auckland, New Zealand

    D. Gleeson

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  1. S. J. Wells
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  2. W. Ji
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  3. S. M. Baillie
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  4. D. Gleeson
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Correspondence to S. J. Wells.

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Wells, S.J., Ji, W., Baillie, S.M. et al. Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae . Conservation Genet Resour 5, 113–116 (2013). https://doi.org/10.1007/s12686-012-9745-z

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  • DOI: https://doi.org/10.1007/s12686-012-9745-z

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