Major histocompatibility complex (MHC) genes can be used to study molecular evolution in wild vertebrate populations. Here, we used “genome walking” to develop primers for amplification of the second exon of a single expressed MHC class II locus in a glass frog, Espadarana prosoblepon. We tested the utility of the primers on two Panamian populations of E. prosoblepon. The MHC marker displayed high levels of allelic diversity (15 alleles/locus identified in each population) and evidence of positive selection in both populations. We also successfully amplified this marker from Sachatamia ilex, another centrolenid species. This marker can be used to assess the evolution of immune genes that may be associated with disease susceptibility in E. prosoblepon and closely related species in Central and South America.
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We thank K.R. Lips for specimen collection and K.E. Tracy for assistance with PCR and cloning. Funding for this study was provided by National Science Foundation Grants (DEB-0815315 and DEB-1120249).
GenBank accession numbers will be provided upon acceptance for publication.
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Kiemnec-Tyburczy, K.M., Zamudio, K.R. Novel locus-specific primers for major histocompatibility complex class II alleles from glass frogs developed via genome walking. Conservation Genet Resour 5, 109–111 (2013). https://doi.org/10.1007/s12686-012-9744-0