Novel locus-specific primers for major histocompatibility complex class II alleles from glass frogs developed via genome walking
Major histocompatibility complex (MHC) genes can be used to study molecular evolution in wild vertebrate populations. Here, we used “genome walking” to develop primers for amplification of the second exon of a single expressed MHC class II locus in a glass frog, Espadarana prosoblepon. We tested the utility of the primers on two Panamian populations of E. prosoblepon. The MHC marker displayed high levels of allelic diversity (15 alleles/locus identified in each population) and evidence of positive selection in both populations. We also successfully amplified this marker from Sachatamia ilex, another centrolenid species. This marker can be used to assess the evolution of immune genes that may be associated with disease susceptibility in E. prosoblepon and closely related species in Central and South America.
KeywordsCentrolenidae Espadarana Immunogenetics Sachatamia
We thank K.R. Lips for specimen collection and K.E. Tracy for assistance with PCR and cloning. Funding for this study was provided by National Science Foundation Grants (DEB-0815315 and DEB-1120249).
- Berger L, Speare R, Daszak P, Green DE, Cunningham AA, Goggin CL, Slocombe R, Ragan MA, Hyatt AD, McDonald KR, Hines HB, Lips KR, Marantelli G, Parkes H (1998) Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America. Proc Natl Acad Sci USA 95:9031–9036PubMedCrossRefGoogle Scholar
- Goudet J (1995) FSTAT (Version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486Google Scholar
- Guayasamin JM, Castroviejo-Fisher S, Trueb L, Ayarzaguena MR, Rada M, Vila C (2009) Phylogenetic systematics of glassfrogs (Amphibia: Centrolenidae) and their sister taxon Allophryne ruthveni. Zootaxa 2100:1–97Google Scholar
- IUCN (2012) IUCN Red list of threatened species. Version 2012.1. http://www.iucnredlist.org. Accessed 05 July 2012
- Klein J (1986) Natural history of the major histocompatibility complex. Wiley, New YorkGoogle Scholar
- Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar