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Development and characterization of twenty-two novel microsatellite markers for the mountain whitefish, Prosopium williamsoni and cross-amplification in the round whitefish, P. cylindraceum, using paired-end Illumina shotgun sequencing

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Abstract

We isolated and characterized a total of 22 microsatellite loci in Prosopium williamsoni. Loci were screened in 24 individuals from across a portion of its range in the Peace River area of northeastern British Columbia. The number of alleles per locus ranged from 3 to 19, observed heterozygosity ranged from 0.111 to 0.950, and the probability of identity values ranged from 0.013 to 0.604. These new loci will be used for conducting investigations into the genetic structure and diversity of extant populations of this important forage fish. The loci were also screened in the congener P. cylindraceum, which is of interest as an indicator species in the Great Lakes region of North America; 15 of the 22 loci produced scorable PCR products in the round whitefish.

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Acknowledgments

Financial support for microsatellite DNA primer development was provided by grants from the BC Hydro and Power Authority and the Natural Sciences and Engineering Research Council of Canada awarded to EBT. We thank Bruce Power and the Canada Research Chairs program for funding to support P. cylindraceum research; C.-L. Fietsch and J. Thompson provided valuable logistic support. Manuscript preparation was partially supported by the DOE under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.

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Authors and Affiliations

  1. Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29802, USA

    Jason O’Bryhim & Stacey L. Lance

  2. Department of Biology, University of Regina, Regina, SK, S4S 0A2, Canada

    Christopher Somers

  3. Department of Zoology, Biodiversity Research Centre and Beaty Biodiversity Museum, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

    Monica Yau & Eric B. Taylor

  4. Bruce Power, Integration Department, Tiverton, ON, N06 2T0, Canada

    Douglas R. Boreham

  5. Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, ON, L8S 4K1, Canada

    Douglas R. Boreham

  6. Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, CO, 80045, USA

    Kenneth L. Jones

Authors
  1. Jason O’Bryhim
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  2. Christopher Somers
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  3. Stacey L. Lance
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  4. Monica Yau
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  5. Douglas R. Boreham
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  6. Kenneth L. Jones
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  7. Eric B. Taylor
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Corresponding author

Correspondence to Eric B. Taylor.

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O’Bryhim, J., Somers, C., Lance, S.L. et al. Development and characterization of twenty-two novel microsatellite markers for the mountain whitefish, Prosopium williamsoni and cross-amplification in the round whitefish, P. cylindraceum, using paired-end Illumina shotgun sequencing. Conservation Genet Resour 5, 89–91 (2013). https://doi.org/10.1007/s12686-012-9740-4

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  • DOI: https://doi.org/10.1007/s12686-012-9740-4

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