Conservation Genetics Resources

, Volume 5, Issue 1, pp 39–41 | Cite as

Isolation and characterisation of polymorphic microsatellite loci for Noisy Miners Manorina melanocephala, with successful cross-amplification in Bell Miners M. melanophrys

  • Anna M. Kopps
  • Paul McDonald
  • Lee Ann Rollins
Technical Note


We characterized 15 polymorphic microsatellite loci identified from a Noisy Miner (Manorina melanocephala) blood sample using 454 whole genome shotgun sequencing. Levels of polymorphism were assessed using 15 Noisy Miners. The average number of alleles per locus was 5.1. These loci were then cross-amplified to assess their suitability in a single population of Bell Miners (M. melanophrys). Given the landscape level impact that these species are having on the health of vegetation and biodiversity of a range of vertebrates throughout much of south-eastern Australia, these primers will help identify colony dispersal patterns and thus aid in modeling predictions of miner presence and tenure length in threatened ecosystems.


Meliphagidae Honeyeaters Microsatellite Population ecology Dispersal Bell Miner associated dieback 



We thank Jason Koval from the Ramaciotti Centre for Gene Function Analysis (University of New South Wales, Sydney, Australia) for performing 454 sequencing. Simon Griffith provided access to molecular facilities at Macquarie University and Nina Svedin assisted with fieldwork. Research was approved by the La Trobe University (AEC01/19(L)/V2) and Macquarie University Animal Ethics Committee (AEC2007/010), the Department of Sustainability and Environment (license 10002082) and the Australian Bird and Bat Banding Scheme (A2259), who also provided leg bands.


  1. Abdelkrim J, Robertson BC, Stanton JL, Gemmell NJ (2009) Fast, cost-effective development of species-specific microsatellite markers by genomic sequencing. Biotechnology 46:185–192CrossRefGoogle Scholar
  2. Arnold KE (2000) Group mobbing behaviour and nest defence in a cooperatively breeding Australian bird. Ethology 106:385–393CrossRefGoogle Scholar
  3. Barrett G, Silcocks A, Barry S, Cunningham R, Poulter R (2003) The new atlas of Australian birds. Shannon Books, MelbourneGoogle Scholar
  4. Haythorpe KM, McDonald PG (2010) Non-lethal foraging by Bell Miners on a herbivorous insect: potential implications for forest health. Austral Ecol 35:444–450CrossRefGoogle Scholar
  5. Higgins PJ, Peter JM, Steele WK (2001) Handbook of Australian, New Zealand and Antarctic Birds. Tyrant-Flycatchers to Chats. Vol 5. Oxford University Press, MelbourneGoogle Scholar
  6. McDonald PG, Kazem AJN, Clarke MF, Wright J (2008) Helping as a signal: does removal of potential audiences alter helper behavior in the Bell Miner? Behav Ecol 19:1047–1055CrossRefGoogle Scholar
  7. Meglécz E, Costedoat C, Dubut V et al (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404PubMedCrossRefGoogle Scholar
  8. Neilan BA, Wilton AN, Jacobs D (1997) A Universal procedure for primer labelling of amplicons. Nucleic Acids Res 25:2938–2939PubMedCrossRefGoogle Scholar
  9. Peakall ROD, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Res 6:288–295Google Scholar
  10. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225CrossRefGoogle Scholar
  11. Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Res 8:103–106CrossRefGoogle Scholar
  12. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
  13. Wardell-Johnson G, Stone C, Recher H, Lynch AJJ (2006) Bell Miner Associated Dieback (BMAD). Independent Scientific Literature Review: A review of eucalypt dieback associated with Bell Miner habitat in north-eastern New South Wales, Australia. DEC NSW Occasional Paper DEC 2006/116Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Anna M. Kopps
    • 1
  • Paul McDonald
    • 1
  • Lee Ann Rollins
    • 1
  1. 1.University of New EnglandArmidaleAustralia

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