Abstract
Ozotoceros bezoarticus is categorized as Threatened in Argentina. In this study, we report 15 microsatellite loci—in the Argentinian populations, 12 are polymorphic and 3 monomorphic—isolated from a dinucleotide enriched genomic library. Among 59 individuals sampled from the four remaining populations in Argentina, the mean number of alleles was 7.58, the expected heterozygosity ranged from 0.33 to 0.88 and the observed heterozygosity from 0.08 to 0.83. Three loci showed deviation from Hardy–Weinberg equilibrium. Some of the loci showed linkage disequilibrium, although there was no coincident pattern for this parameter in all populations. The primers reported here constitute the first specific set for the species, and their use will be invaluable to generate the necessary information on genetic diversity in order to help conservation plans.
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Acknowledgments
We thank Buenos Aires and Corrientes provincial’s Environmental Agencies; Alejandro Larriera and Eduardo Mosso from Dirección General de Manejo Sustentable de Fauna y Flora—Secretaría de Medio Ambiente (MASPyMA) Santa Fe, Conservation Lund Trust and Fundación Vida Silvestre Argentina for providing logistics and economic resources and principally for their support with sample collection. We also thank Andrés Pautasso, Lorena Perez Carusi, Mariano Merino, Marcela Uhart and Manuel De María. We are grateful to Provincial and Nationals Rangers Mario Beade and Eduardo Gómez, and to Cecilia Li Puma from the Secretaría de Medio Ambiente de la Nación. Thanks also to Museo Provincial de Ciencias Naturales Florentino Ameghino, Santa Fe and Museo Nacional de Ciencias Naturales Bernardino Rivadavia. We thank Anete Pereira de Souza from UNICAMP, Brasil, for her technical support developing the enriched library. This research was supported by a grant from CONICET (PIP-0329).
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Raimondi, V.B., Maruyama Mori, G., Piedrabuena, M.R. et al. Isolation and characterization of fifteen microsatellite loci from the endangered pampas deer (Ozotoceros bezoarticus, Cervidae). Conservation Genet Resour 4, 1089–1092 (2012). https://doi.org/10.1007/s12686-012-9712-8
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DOI: https://doi.org/10.1007/s12686-012-9712-8